Low-metastatic melanoma cells acquire enhanced metastatic capability via exosomal transfer of miR-199a-1-5p from highly metastatic melanoma cells

作者全名："Zhao, Qiting; Chen, Hao; Li, Xiaoshuang; Zeng, Bin; Sun, Zhiwei; Liu, Doudou; Chen, Yuting; Zhang, Yuhan; Xing, H. Rosie; Wang, Jianyu"

作者地址："[Zhao, Qiting; Chen, Hao; Li, Xiaoshuang; Liu, Doudou; Chen, Yuting; Zhang, Yuhan; Xing, H. Rosie] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China; [Zhao, Qiting; Chen, Hao; Li, Xiaoshuang; Liu, Doudou; Chen, Yuting; Zhang, Yuhan; Xing, H. Rosie] Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China; [Zhao, Qiting; Chen, Hao; Zeng, Bin; Sun, Zhiwei; Wang, Jianyu] Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China"

通信作者："Xing, HR (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China.; Xing, HR (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China.; Wang, JY (通讯作者)，Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China."

来源：CELL DEATH DISCOVERY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000781379100002

JCR分区：Q2

影响因子：7

年份：2022

卷号：8

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："The mean survival of metastatic melanoma is less than 1 year. While the high mortality rate is associated with the efficient metastatic colonization of the involved organs, the underlying mechanisms remain elusive. The role of exosomes in facilitating the interactions between cancer cells and the metastatic microenvironment has received increasing attention. Previous studies on the role of exosomes in metastasis have been heavily focused on cancer cell-derived exosomes in modulating the functions of stromal cells. Whether the extravasated neighboring cancer cells at the distant organ can alter the metastatic properties of one another, a new mechanism of metastatic colonization, has not been demonstrated prior to this report. In this study, a paired M4 melanoma derivative cell lines, i.e., M14-OL and POL, that we established and characterized were employed. They exhibit high (POL cells) and low (OL cells) metastatic colonization efficiency in vivo, respectively. We show that exosomal crosstalk between metastatic cancer cells is a new mechanism that underlies cancer metastasis and heterogeneity. Low metastatic melanoma cells (OL) can acquire the ""metastatic power"" from highly metastatic melanoma cells (POL). POL achieves this goal by utilizing its exosomes to deliver functional miRNAs, such as miR-199a-1-5p, to the targeted OL cell which in turn inactivates cell cycle inhibitor CDKN1B and augments metastatic colonization."

基金机构："National Natural Science Fund [82073277, 81672908]; Science and Technology Project Affiliated to the Education Department of Chongqing [KJQN201800426]; Natural Science Fund of Chongqing [cstc2019jcyj-msxmX0868]; Science and Technology Project of Chongqing Yuzhong District [20200110]"

基金资助正文："This work was supported by the National Natural Science Fund (Grant No. 82073277 and 81672908), the Science and Technology Project Affiliated to the Education Department of Chongqing (Grant No. KJQN201800426), Natural Science Fund of Chongqing (Grant No. cstc2019jcyj-msxmX0868) and Science and Technology Project of Chongqing Yuzhong District (Grant No. 20200110)."