miR-489-3p promotes malignant progression of non-small cell lung cancer through the inactivation of Wnt/beta-catenin signaling pathway via regulating USP48

作者全名："Zhang, Pei; Li, Li; Wang, Bing; Ran, Xu; Yang, Shengrong; Luo, Yujie; Li, Yunhe; Wang, Zhenghong; Liu, Yi; Zhu, Bing"

作者地址："[Zhang, Pei; Li, Li; Wang, Bing; Ran, Xu; Yang, Shengrong; Luo, Yujie; Li, Yunhe; Wang, Zhenghong; Liu, Yi; Zhu, Bing] Chongqing Med Univ, Dept Thorac & Cardiovasc Surg, Affiliated Hosp 2, 76 Linjiang Rd, Chongqing 400010, Peoples R China"

通信作者："Zhu, B (通讯作者)，Chongqing Med Univ, Dept Thorac & Cardiovasc Surg, Affiliated Hosp 2, 76 Linjiang Rd, Chongqing 400010, Peoples R China."

来源：RESPIRATORY RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000781918200001

JCR分区：Q1

影响因子：5.8

年份：2022

卷号：23

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：NSCLC; miR-489-3p; USP48; Wnt/beta-catenin signaling pathway

摘要："Background: Non-small cell lung cancer (NSCLC) is the most prevalent form of lung cancer globally, with average age of cancer patients becoming younger gradually. It is of significance to gain a comprehensive understanding of molecular mechanism underlying NSCLC. Methods: Quantitative polymerase chain reaction (qPCR) and western blot were applied to measure RNA and protein levels separately. Functional assays and western blot were performed to determine the effects of miR-489-3p and USP48 on cell growth, migration and epithelial-mesenchymal transition (EMT) in NSCLC. TOP/FOP flash luciferase reporter assay was carried out to detect the activity of Wnt pathway. Besides, qPCR, RNA pulldown and luciferase reporter assays were conducted to probe into the target gene of miR-489-3p. Immunoprecipitation-western blot (IP-western blot) analysis was implemented to assess the effect of USP48 on the ubiquitination of beta-catenin. Results: miR-489-3p hampers NSCLC cell proliferation, migration and EMT in vitro and NSCLC tumorigenesis and metastasis in vivo. Additionally, miR-489-3p inactivates Wnt/beta-catenin signaling pathway and regulates USP48 to inhibit the ubiquitination of beta-catenin. Moreover, USP48 propels the development of NSCLC cells. Conclusions: The current study demonstrated that miR-489-3p promotes the malignant progression of NSCLC cells via targeting USP48, which might offer a new perspective into NSCLC treatment."

基金机构：Reserved Candidates Training Project for Academic and Technical Leaders of Chongqing; High-level Medical Reserved Personnel Training Project of Chongqing; Backbone Members Supporting Project of the Second Affiliated Hospital of Chongqing Medical University; Outstanding Young Talents supporting Project of the Second Affiliated Hospital of Chongqing Medical University

基金资助正文：This paper was funded by Reserved Candidates Training Project for Academic and Technical Leaders of Chongqing; High-level Medical Reserved Personnel Training Project of Chongqing; Backbone Members Supporting Project of the Second Affiliated Hospital of Chongqing Medical University; Outstanding Young Talents supporting Project of the Second Affiliated Hospital of Chongqing Medical University.