One-step isothermal amplification strategy for microRNA specific and ultrasensitive detection based on nicking-assisted entropy-driven DNA circuit triggered exponential amplification reaction

作者全名："Yuan, Changjing; Fang, Jie; Luo, Xizi; Zhang, Yang; Huang, Guorong; Zeng, Xiaojun; Xia, Ke; Li, Mengya; Chen, Xueping; Yang, Xiang; de la Chapelle, Marc Lamy; Fu, Weiling"

作者地址："[Yuan, Changjing; Fang, Jie; Luo, Xizi; Huang, Guorong; Zeng, Xiaojun; Xia, Ke; Li, Mengya; Yang, Xiang; Fu, Weiling] Army Med Univ, Third Mil Med Univ, Southwest Hosp, Dept Lab Med, Chongqing 400038, Peoples R China; [de la Chapelle, Marc Lamy] Univ Mans, Inst Mol & Materiaux Mans, IMMM UMR CNRS 6283, Ave Olivier Messiaen, F-72085 Le Mans, France; [Zhang, Yang] Chongqing Univ Canc Hosp, Dept Lab Med, Chongqing 400030, Peoples R China; [Chen, Xueping] Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing 400016, Peoples R China"

通信作者："Yang, X; Fu, WL (通讯作者)，Army Med Univ, Third Mil Med Univ, Southwest Hosp, Dept Lab Med, Chongqing 400038, Peoples R China.; de la Chapelle, ML (通讯作者)，Univ Mans, Inst Mol & Materiaux Mans, IMMM UMR CNRS 6283, Ave Olivier Messiaen, F-72085 Le Mans, France."

来源：ANALYTICA CHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:000782581200009

JCR分区：Q1

影响因子：6.2

年份：2022

卷号：1203

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Exponential amplification reaction; Fluorescence; Isothermal amplification; MicroRNA; Nicking-assisted entropy-driven reaction

摘要："Sensitive and specific detection of microRNAs (miRNAs) is of critical significance for early diagnosis of cancers such as pancreatic cancer with atypical initial symptoms and high mortality. Despite exponential amplification reaction (EXPAR) is an attractive isothermal amplification method for detecting miRNAs, it faces the problems of the dependence difference and low specificity. To address such challenges, herein, a nicking-assisted entropy-driven DNA circuit triggered exponential amplification reaction (NAED-EXPAR)"

基金机构："National Natural Science Founda-tion of China [81920108024, 82172374]; Military logistics scientific research project [AWS17J010]"

基金资助正文："Acknowledgements This work was supported by National Natural Science Founda-tion of China (81920108024, 82172374) and the Military logistics scientific research project (AWS17J010) ."