Study on the physicochemical properties and immunomodulatory anti-tumor effect of the Pholiota adiposa polysaccharide

作者全名："Yang, Zhongwei; Liu, Zijing; Xu, Jie; Zhu, Junmo; Pu, Youwei; Bao, Yixi"

作者地址："[Yang, Zhongwei; Xu, Jie; Zhu, Junmo; Pu, Youwei; Bao, Yixi] Chongqing Med Univ, Dept Clin Lab, Affliated Hosp 2, Chongqing 400010, Peoples R China; [Liu, Zijing] Chongqing Med Univ, Dept Gastroenterol, Affliated Hosp 3, Chongqing 400010, Peoples R China"

通信作者："Bao, YX (通讯作者)，Chongqing Med Univ, Dept Clin Lab, Affliated Hosp 2, Chongqing 400010, Peoples R China."

来源：FOOD & FUNCTION

ESI学科分类：AGRICULTURAL SCIENCES

WOS号：WOS:000782938100001

JCR分区：Q1

影响因子：6.1

年份：2022

卷号：13

期号：9

开始页：5153

结束页：5165

文献类型：Article

关键词：　

摘要："In this study, the extraction, purification, physical and chemical properties, and biological activity of the Pholiota adiposa (PAP) polysaccharide were investigated. One fraction (PAP-1a) of Pholiota adiposa polysaccharides was isolated using DEAE Sepharose (TM) Fast Flow and Sephacryl (TM) S-300 High-Resolution columns. The HPLGPC results revealed that the molecular weight of PAP-1a was 16.453 kDa. PAP-1a was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose, and fucose and their molar % was 33.41, 0.53, 1.33, 0.07, 0.27, 5.28, 38.31, 0.83, 18.04 and 2.23, respectively. PAP-1a could activate macrophages to secrete NO and cytokines such as TNF-a, IL-6, and IL-12p70. When hepatocellular carcinoma cells (HCCs) and macrophages were co-cultured, it was observed that PAP-1a inhibited the growth of Hep-G2, Hep-3B, and Huh7 via immunoregulation. It triggered cell apoptosis by blocking the cell cycle in the G0/G1 stage. Furthermore, PAP-1a had no direct cytotoxicity against the hepatocyte cell line L02 and macrophages RAW264.7."

基金机构："National Natural Science Foundation of China [81773940, 81473388]; Natural Science Foundation of Chongqing Province of China [cstc2021jcyj-msxmX0094]"

基金资助正文：This research was supported by the National Natural Science Foundation of China (No. 81773940 and 81473388) and the Natural Science Foundation of Chongqing Province of China (No. cstc2021jcyj-msxmX0094).