Low-intensity pulsed ultrasound enhances bone marrow-derived stem cells-based periodontal regenerative therapies

作者全名："Wang, Yunji; Li, Jie; Zhou, Jianpin; Qiu, Ye; Song, Jinlin"

作者地址："[Qiu, Ye; Song, Jinlin] Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China; Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; Chongqing Municipal Key Lab Oral Biomed Engn High, Chongqing, Peoples R China"

通信作者："Qiu, Y; Song, JL (通讯作者)，Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China."

来源：ULTRASONICS

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000783921700002

JCR分区：Q1

影响因子：4.2

年份：2022

卷号：121

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Bone marrow stromal cells; Low-intensity pulsed ultrasound; Cell homing; Alveolar bone

摘要："Background: Alveolar bone loss is one of the most common consequence for periodontitis, which is a major obstacle in periodontal regeneration. Bone marrow stromal cells (BMSCs) have shown significant promise in the treatment of various disease, which also contribute to the natural bone repair process. Low-intensity pulsed ultrasound (LIPUS) is a therapeutic ultrasound used in our previous studies to promotes alveolar bone regeneration. In addition, LIPUS was found to be a promising method to enhance mesenchymal stromal cell-based therapies. In the current study, we have investigated the effects of LIPUS combined with BMSCs therapies on BMSCs homing and its potential to promote alveolar bone regeneration. Methods: BMSCs were isolated from rat and characterized by multilineages differentiation assay. Then these cells were labeled with luciferase and green fluorescent protein (GFP) by lentivirus in vitro. Periodontal bone defect was made on the mesial area of the maxillary first molar in rats. A total of 1 x 106 Luc-GFP labeled BMSCs were injected into rat tail vein. Bioluminescence imaging was utilized to track BMSCs in vivo. The rats were sacrificed eight weeks after surgery and the samples were harvested. Micro-computed tomography (Micro-CT) was performed to evaluate alveolar bone regeneration. Paraffin sections were made and subject to hematoxylin-eosin staining, masson staining and immunohistochemistry staining. Results: BMSCs display a fibroblast-like morphology and can differentiate into adipocytes or osteoblasts under appropriate condition. The transfected BMSCs are strongly positive for GFP express. Bioluminescence imaging showed that most of BMSCs were trapped in the lung. A small portion BMSCs were homed to the alveolar bone defect area in BMSCs group, while more cells were observed in BMSCs/LIPUS group compare to other groups on day 3 and 7. Micro-CT results showed that BMSCs/LIPUS group resulted in more new bone formation than other groups. Immunohistochemical results showed higher expression of COL-I and osteopontin in BMSCs/LIPUS group compared with the other groups. Conclusions: These results suggested that LIPUS can enhance BMSCs-based periodontal alveolar bone regeneration. This study provides new insights into how LIPUS might provide therapeutic benefits by promoting BMSCs homing."

基金机构："National Natural Science Foundation of China [81901007, 31971282, 82071072]; Natural Science Foundation of Chongqing [cstc2019jcyj-msxmX0854]; Chongqing Graduate Tutor Team"

基金资助正文："Acknowledgements This work was supported by National Natural Science Foundation of China (No. 81901007, 31971282, 82071072) , and Natural Science Foundation of Chongqing (No. cstc2019jcyj-msxmX0854) , and Chongqing Graduate Tutor Team (2019) . We are thankful to doctor Yan Wu for providing language help."