LIFU-responsive nanomedicine enables acoustic droplet vaporization-induced apoptosis of macrophages for stabilizing vulnerable atherosclerotic plaques

作者全名："Hou, Jingxin; Zhou, Jun; Chang, Meiqi; Bao, Guangcheng; Xu, Jie; Ye, Man; Zhong, Yixin; Liu, Shuling; Wang, Junrui; Zhang, Wei; Ran, Haitao; Wang, Zhigang; Chen, Yu; Guo, Dajing"

作者地址："[Hou, Jingxin; Zhou, Jun; Xu, Jie; Ye, Man; Zhong, Yixin; Liu, Shuling; Wang, Junrui; Zhang, Wei; Guo, Dajing] Chongqing Med Univ, Dept Radiol, Affiliated Hosp, Chongqing 400010, Peoples R China; [Chen, Yu] Shanghai Univ, Sch Life Sci, Materdicine Lab, Shanghai 200444, Peoples R China; [Ran, Haitao; Wang, Zhigang] Chongqing Med Univ, Chongqing Key Lab Ultrasound Mol Imaging, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Ran, Haitao; Wang, Zhigang] Chongqing Med Univ, Dept Ultrasound, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Chang, Meiqi] Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China; [Bao, Guangcheng] Chongqing Med Univ, Dept Radiol, Childrens Hosp, Chongqing 400014, Peoples R China"

通信作者："Guo, DJ (通讯作者)，Chongqing Med Univ, Dept Radiol, Affiliated Hosp, Chongqing 400010, Peoples R China.; Chen, Y (通讯作者)，Shanghai Univ, Sch Life Sci, Materdicine Lab, Shanghai 200444, Peoples R China.; Wang, ZG (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Ultrasound Mol Imaging, Affiliated Hosp 2, Chongqing 400010, Peoples R China.; Wang, ZG (通讯作者)，Chongqing Med Univ, Dept Ultrasound, Affiliated Hosp 2, Chongqing 400010, Peoples R China."

来源：BIOACTIVE MATERIALS

ESI学科分类：　

WOS号：WOS:000788758800001

JCR分区：Q1

影响因子：18.9

年份：2022

卷号：16

期号：　

开始页：120

结束页：133

文献类型：Article

关键词：Vulnerable plaques; Acoustic droplet vaporization; Macrophages; Apoptosis; Nanomedicine

摘要："Due to the high risk of tearing and rupture, vulnerable atherosclerotic plaques would induce serious cardiovascular and cerebrovascular diseases. Despite the available clinical methods can evaluate the vulnerability of plaques and specifically treat vulnerable plaques before a cardiovascular event, but the efficiency is still low and undesirable. Herein, we rationally design and engineer the low-intensity focused ultrasound (LIFU)-responsive FPD@CD nanomedicine for the highly efficient treatment of vulnerable plaques by facilely loading phase transition agent perfluorohexane (PFH) into biocompatible PLGA-PEG-PLGA nanoparticles (PPP NPs) and then attaching dextran sulphate (DS) onto the surface of PPP NPs for targeting delivery. DS, as a typical macrophages-targeted molecule, can achieve the precise vaporization of NPs and subsequently controllable apoptosis of RAW 264.7 macrophages as induced by acoustic droplet vaporization (ADV) effect. In addition, the introduction of DiR and Fe3O4 endows nanomedicine with near-infrared fluorescence (NIRF) and magnetic resonance (MR) imaging capabilities. The engineered FPD@CD nanomedicine that uses macrophages as therapeutic targets achieve the conspicuous therapeutic effect of shrinking vulnerable plaques based on in vivo and in vitro evaluation outcomes. A reduction of 49.4% of vascular stenosis degree in gross pathology specimens were achieved throughout the treatment period. This specific, efficient and biosafe treatment modality potentiates the biomedical application in patients with cardiovascular and cerebrovascular diseases based on the relief of the plaque rupture concerns."

基金机构："Na-tional Natural Science Foundation of China [81701650, 81971608, 82172092]; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University [2020-7]; Science & Technology Commission Foundation of Chongqing [cstc2017jcyjAX0444]"

基金资助正文："The authors gratefully acknowledge financial support from the Na-tional Natural Science Foundation of China (Grant Nos. 81701650, 81971608, and 82172092) , the Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University (Grant No. 2020-7) and the Science & Technology Commission Foundation of Chongqing (Grant No. cstc2017jcyjAX0444) ."