A new HClO-activated "turn-off" mitochondria-targetable NIR fluorescent probe for imaging of osteoarthritis in vivo

作者全名："Luo, Quan; Luo, Ziyi; Zeng, Hui; Xiao, Yu; Peng, Yongbo; Liu, Gang"

作者地址："[Luo, Quan; Luo, Ziyi; Zeng, Hui; Xiao, Yu; Liu, Gang] Southern Med Univ, Dept Rehabil Med, Affilicated Hosp 3, Guangzhou 510500, Peoples R China; [Liu, Gang] Southern Med Univ, Nanfang Hosp, Dept Rehabil Med, Guangzhou 510515, Peoples R China; [Peng, Yongbo] Chongqing Med Univ, Coll Pharm, Chongqing Key Lab Pharmaceut Metab Res, Key Lab Biochem & Mol Pharmacol, Chongqing 400016, Peoples R China"

通信作者："Peng, YB; Liu, G (通讯作者)，Southern Med Univ, Dept Rehabil Med, Affilicated Hosp 3, Guangzhou 510500, Peoples R China."

来源：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

ESI学科分类：CHEMISTRY

WOS号：WOS:000788837700015

JCR分区：Q1

影响因子：4.4

年份：2022

卷号：273

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：NIR; HClO; Fluorescence imaging; Osteoarthritis

摘要："Hypochlorous acid (HClO) as a biomarker of inflammation has been implicated in redox signaling and combating microbial infection. Therefore, it is of great significance to develop an efficient method for detection and analysis of HClO in osteoarthritis. Herein, a new""turn-off"" mitochondria-targetable NIR fluorescent probe, NIR-ClO, was reported for specific analysis and imaging of osteoarthritis response-related HOCl levels in vitro and in vivo. In the presence of HClO, due to the specific HOCl-triggered C = C bond cleavage reaction, NIR-ClO obtained a high sensitive and selective fluorescence ""On-Off"" response toward HClO with a good limit of detection(LOD) as low as 28.3 nM, and showed a fast response time (<60 s) , which allow it to be used for detection of HClO under a simulated physiological condition. In addition, NIR-ClO was successfully used to imaging of HClO in living RAW264.7 cells and osteoarthritis model rat. The results suggest that NIR-ClO is a robust tool for future studies on diagnosis osteoarthritis. (c) 2022 Elsevier B.V. All rights reserved."

基金机构：Hunan Provincial Health Commission [202203102522]

基金资助正文：This work was partially supported by Hunan Provincial Health Commission (202203102522).