A dual-signal electrochemical immunosensor for the detection of HPV16 E6 oncoprotein based on PdBP dendritic ternary nanospheres and MBSi-Chi nanocomposites

作者全名："Wen, Tao; Xia, Chenchen; Yu, Qiubo; Yu, Yujie; Li, Siyuan; Zhou, Chunli; Sun, Kexin; Yue, Song"

作者地址："[Wen, Tao; Xia, Chenchen; Li, Siyuan; Zhou, Chunli] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China; [Yu, Qiubo] Chongqing Med Univ, Mol Med Lab, Chongqing 400016, Peoples R China; [Yu, Qiubo] Chongqing Med Univ, Dept Pathol, Chongqing 400016, Peoples R China; [Yu, Yujie] Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 400016, Peoples R China; [Sun, Kexin] Chongqing Med Univ, Chongqing Eye Inst, Dept Ophthalmol, Chongqing Key Lab Ophthalmol,Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Yue, Song] Chongqing Med Univ, Obstet & Gynecol Dept, Affiliated Hosp 2, Chongqing 400016, Peoples R China"

通信作者："Sun, KX (通讯作者)，Chongqing Med Univ, Chongqing Eye Inst, Dept Ophthalmol, Chongqing Key Lab Ophthalmol,Affiliated Hosp 1, Chongqing 400016, Peoples R China.; Yue, S (通讯作者)，Chongqing Med Univ, Obstet & Gynecol Dept, Affiliated Hosp 2, Chongqing 400016, Peoples R China."

来源：ANALYST

ESI学科分类：CHEMISTRY

WOS号：WOS:000790834400001

JCR分区：Q1

影响因子：4.2

年份：2022

卷号：147

期号：10

开始页：2272

结束页：2279

文献类型：Article

关键词：　

摘要："Cervical cancer is a common cancer in women. HPV16 E6 oncoprotein is a reliable biomarker for cervical cancer. Although there are other methods for detecting E6 oncoprotein, the electrochemical method has more advantages, such as low cost, convenience and speed. In this study, a novel dual-signal electrochemical immunosensor for quick and sensitive detection of E6 oncoprotein based on a high efficiency catalyst and signal label was developed. Herein, to achieve quick detection, palladium-boron-phosphorus dendritic ternary nanospheres (PdBP NSs) not only acted as a catalyst to catalyze H2O2, but also as a support material to capture antibodies. Moreover, to realize sensitive detection, nanocomposites of mesoporous silica nanoparticles loaded with methylene blue and coated with chitosan (MBSi-Chi) were synthesized as a signal label, which can produce electrochemical signal. Under optimal conditions, the label-free immunosensor exhibited a linear range of 100 fg mL(-1) to 4 ng mL(-1) with a detection limit of 72.8 fg mL(-1) (S/N = 3), and the sandwich-type immunosensor presented a linear range of 50 fg mL(-1) to 4 ng mL(-1) with a detection limit of 34.1 fg mL(-1) (S/N = 3). The as-prepared dual-signal immunosensor had desirable specificity, stability and repeatability, implying its potential applications in clinical laboratory."

基金机构：National Natural Science Foundation of China [81100443]; Chongqing Science and Technology Committee Project [cstc2019jscxmsxmX0246]; Chongqing Municipal Health Bureau Scientific Research Project [20132151]

基金资助正文："We would like to thank the National Natural Science Foundation of China (grant no. 81100443), the Chongqing Science and Technology Committee Project (c stc2019jscxmsxmX0246) and the Chongqing Municipal Health Bureau Scientific Research Project (grant no. 20132151) for supporting this study."