Surface modification of titanium substrate via combining photothermal therapy and quorum-sensing-inhibition strategy for improving osseointegration and treating biofilm-associated bacterial infection

作者全名："Hu, Jingwei; Ding, Yao; Tao, Bailong; Yuan, Zhang; Yang, Yulu; Xu, Kun; Li, Xuan; Liu, Peng; Cai, Kaiyong"

作者地址："[Hu, Jingwei; Ding, Yao; Yang, Yulu; Xu, Kun; Li, Xuan; Liu, Peng; Cai, Kaiyong] Chongqing Univ, Coll Bioengn, Key Lab Biorheol Sci & Technol, Minist Educ, Chongqing 400044, Peoples R China; [Tao, Bailong] Chongqing Med Univ, Lab Res Ctr, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Yuan, Zhang] Northwestern Polytech Univ, Inst Med Res, Xian 710072, Peoples R China"

通信作者："Liu, P; Cai, KY (通讯作者)，Chongqing Univ, Coll Bioengn, Key Lab Biorheol Sci & Technol, Minist Educ, Chongqing 400044, Peoples R China."

来源：BIOACTIVE MATERIALS

ESI学科分类：　

WOS号：WOS:000791151500002

JCR分区：Q1

影响因子：18.9

年份：2022

卷号：18

期号：　

开始页：228

结束页：241

文献类型：Article

关键词：Titanium implant; Biofilm infection; Osseointegration; Photothermal therapy; Quorum sensing

摘要："Insufficient osseointegration and biofilm-associated bacterial infection are important challenges for clinical application of titanium (Ti)-based implants. Here, we constructed mesoporous polydopamine (MPDA) nanoparticles (NPs) loaded with luteolin (LUT, a quorum sensing inhibitor), which were further coated with the shell of calcium phosphate (CaP) to construct MPDA-LUT@CaP nanosystem. Then, MPDA-LUT@CaP NPs were immobilized on the surface of Ti implants. Under acidic environment of bacterial biofilm-infection, the CaP shell of MPDA-LUT@CaP NPs was rapidly degraded and released LUT, Ca2+ and PO43- from the surface of Ti implant. LUT could effectively inhibit and disperse biofilm. Furthermore, under near-infrared irradiation (NIR), the thermotherapy induced by the photothermal conversion effect of MPDA destroyed the integrity of the bacterial membrane, and synergistically led to protein leakage and a decrease in ATP levels. Combined with photothermal therapy (PTT) and quorum-sensing-inhibition strategy, the surface-functionalized Ti substrate had an antibacterial rate of over 95.59% against Staphylococcus aureus and the elimination rate of the formed biofilm was as high as 90.3%, so as to achieve low temperature and efficient treatment of bacterial biofilm infection. More importantly, the modified Ti implant accelerated the growth of cell and the healing process of bone tissue due to the released Ca2+ and PO43-. In summary, this work combined PIT with quorum-sensing-inhibition strategy provides a new idea for surface functionalization of implant for achieving effective antibacterial and osseointegration capabilities."

基金机构："National Natural Science Foundation of China [32171327, 21734002, 51825302]; Natural Science Foundation of Chongqing [cstc2021jcyj-cxttX0002]"

基金资助正文："This work was financially supported by National Natural Science Foundation of China (32171327, 21734002 and 51825302) , and the Natural Science Foundation of Chongqing (cstc2021jcyj-cxttX0002) . The Analytical and Testing Center of Chongqing University is greatly acknowledged for the help with the characterization of materials."