Chromogranin A-derived peptide CGA47-66 protects against septic brain injury by reducing blood-brain barrier damage through the PI3K/AKT pathway

作者全名："Wang, FengLin; Zeng, Yan; Liu, Xian; Cao, JiaJun; Kang, ShengNan; Zhou, WuShuang; Chen, XiaoYing; Liu, JingLun; Zhang, Dan"

作者地址："[Wang, FengLin; Zeng, Yan; Liu, Xian; Cao, JiaJun; Kang, ShengNan; Zhou, WuShuang; Liu, JingLun; Zhang, Dan] Chongqing Med Univ, Dept Emergency, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Zeng, Yan] Chongqing Univ, Intens Care Unit, Cent Hosp, Chongqing 400016, Peoples R China; [Chen, XiaoYing] Chongqing Med Univ, Dept Surg Care Unit, Affiliated Hosp 1, Chongqing 400016, Peoples R China"

通信作者："Zhang, D (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Emergency Dept, 12th Floor,Bldg 5A,1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000791299100010

JCR分区：Q2

影响因子：3.1

年份：2022

卷号：605

期号：　

开始页：162

结束页：170

文献类型：Article

关键词：Septic brain injury; Blood-brain barrier; Cognitive impairment; <p>PI3K/AKT</p>; CGA(47-66)

摘要："CGA(47-66) (Chromofungin, CHR), is a peptide derived from the N-terminus of chromogranin A (CgA), has been proven to inhibit the lipopolysaccharide (LPS)-induced brain injury. However, the underlying mechanism is still unknown. We found that CGA(47-66) exerted a protective effect on cognitive impairment by inhibiting the destruction of the blood-brain barrier (BBB) in the LPS-induced sepsis mice model. In addition, the hCMEC/D3 cell line was used to establish an in vitro BBB model. Under LPS stimulation, CGA(47-66 )could significantly alleviate the hyperpermeability of the BBB, the destruction of tight junction proteins, and the rearrangement of F-actin. To investigate the underlying mechanism, we used LY294002, a PI3K inhibitor, which partially reduced the protective effect of CGA(47-66) on the integrity of BBB. Indicating that the PI3K/AKT pathway plays a vital role in the brain-protective function of CGA(47-66), which might be a potential therapeutic target for septic brain injury. (C)& nbsp;2022 Elsevier Inc. All rights reserved."

基金机构：　

基金资助正文：