Treg/Th17 Ratio Regulation May Play an Important Role in Epigallocatechin-3-Gallate-Mediated Attenuation of Increased Afterload-Induced Cardiac Hypertrophy

作者全名："Luo, Min; Mou, Qiuhong; Liu, Lingjuan; Tian, Jie; Liu, Lifei"

作者地址："[Luo, Min; Mou, Qiuhong; Liu, Lingjuan; Tian, Jie; Liu, Lifei] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Chongqing Key Lab Pediat,Childrens Hosp, Minist Educ,Key Lab Child Dev & Disorders,Dept Ca, Chongqing, Peoples R China; [Liu, Lifei] Chongqing Med Univ, Dept Anesthesiol, Childrens Hosp, Chongqing, Peoples R China"

通信作者："Tian, J; Liu, LF (通讯作者)，Chongqing Med Univ, Childrens Hosp, 136 Zhongshan Er Rd, Chongqing 400015, Peoples R China."

来源：JOURNAL OF CARDIOVASCULAR PHARMACOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000791503600015

JCR分区：Q2

影响因子：3

年份：2022

卷号：79

期号：5

开始页：711

结束页：718

文献类型：Article

关键词：cardiac hypertrophy; epigallocatechin-3-gallate; increased afterload; Treg; Th17

摘要："The aim of this study was to investigate whether Treg/Th17 ratio regulation plays an important role in epigallocatechin-3-gallate (EGCG) in attenuating increased afterload-induced cardiac hypertrophy. Three-month-old male C57BL/6 mice were divided into sham + vehicle, abdominal aortic constriction (AAC) + vehicle, and AAC + EGCG groups. Intraperitoneal EGCG (50 mg/kg/d) administration was conducted. Cardiac structure and function were examined by ultrasonography. Pathology was examined by hematoxylin and eosin staining, wheat germ agglutinin staining, and Masson's trichome staining. T-lymphocyte subtypes were analyzed using immunofluorescence and flow cytometry assays. Ultrasonography showed that the ventricular wall in the AAC + vehicle group was thicker than that in the sham + vehicle group (P < 0.05). Hematoxylin and eosin staining revealed cardiomyocyte hypertrophy accompanied by a small amount of inflammatory cell infiltration in the AAC + vehicle group. The results of wheat germ agglutinin staining demonstrated the presence of hypertrophic cardiomyocytes in the AAC + vehicle group (P < 0.01). Masson's trichome staining showed cardiac fibrosis in the AAC + vehicle group, and the immunofluorescence assay revealed infiltration of CD4(+) cells in both AAC + vehicle and AAC + EGCG groups. Splenic flow cytometry showed a significant increase in the proportion of Treg cells in the AAC + EGCG group (P < 0.05). The proportion of Th17 cells in the AAC + vehicle group was significantly higher than that in the sham + vehicle group (P < 0.05). In conclusion, changes in the Treg/Th17 ratio are associated with the occurrence of myocardial hypertrophy caused by increased afterload. Moreover, regulation of the Treg/Th17 ratio by EGCG may play an important role in the attenuation of myocardial hypertrophy."

基金机构："Natural Science Foundation of Chongqing, China [cstc2019jcyj-msxmX0867]"

基金资助正文："Supported by the Natural Science Foundation of Chongqing, China. Grant/Award Numbers: cstc2019jcyj-msxmX0867."