Drug-loaded nanoparticles conjugated with genetically engineered bacteria for cancer therapy

作者全名："Du, Yan; Lin, Li; Zhang, Zhong; Tang, Yu; Ou, Xia; Wang, Yaotai; Zou, Jianzhong"

作者地址："[Du, Yan; Lin, Li; Zhang, Zhong; Tang, Yu; Ou, Xia; Wang, Yaotai; Zou, Jianzhong] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China; [Du, Yan] Chongqing Univ, Peoples Hosp Chongqing 4, Ultrasonog Dept, Cent Hosp, Chongqing 400014, Peoples R China"

通信作者："Zou, JZ (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China."

来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000791590000006

JCR分区：Q2

影响因子：3.1

年份：2022

卷号：606

期号：　

开始页：29

结束页：34

文献类型：Article

关键词：Nanoparticles; Gas vesicles; High intensity focused ultrasound; Chemotherapy

摘要："Drug-loaded nanoparticles have been widely used as synergists in high-intensity focused ultrasound (HIFU) tumor ablation therapy. However, these synergists have certain limitations, such as poor tumor targeting and low accumulation at the tumor site, that restrict the therapeutic efficacy of HIFU. In this study, we utilized drug-loaded nanoparticles conjugated with genetically engineered bacteria which can selectively colonize the hypoxic areas of tumor to facilitate HIFU ablation. Genetically modified Escherichia coil carrying gas vesicles (GVs-E. coli), which were gas-filled protein nanostructures, had a negatively charged surface and could specifically target into the tumor. In contrast, paclitaxel (PTX) and perfluorohexane (PFH) co-loaded cationic lipid nanoparticles (PTX-CLs) had a positively charged surface, hence, GVs-E. coli was used as a vehicle by conjugating with PTX-CLs via electrostatic adsorption and subsequently attracting more PTX-CLs to the tumor site. To improve the therapeutic efficiency of HIFU, the GVs in GVs-E. coli. and PFH encapsulated in PTX-CLs could act as cavitation nuclei to enhance the HIFU cavitation effect, while PTX entrapped in PTX-CLs was released at the tumor site under HIFU irradiation, enhancing the therapeutic efficacy of HIFU and chemo-synergistic therapy. This novel combination strategy has great potential for cancer treatment. (C) 2022 Elsevier Inc. All rights reserved."

基金机构："State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, People's Republic of China"

基金资助正文："The authors are greatly thankful for the financial support by State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, 400016, People's Republic of China."