Preventive effects of traditional Chinese medicine formula Huoxiangzhengqi against lipopolysaccharide-induced inflammatory response

作者全名："Gao, Min; Zou, Zhen; Qiu, Yu; Sumayyah, Golamaully; Jiang, Xuejun; Su, Junhao; Duan, Xinhao; Chen, Chengzhi; Qiu, Jingfu"

作者地址："[Gao, Min; Su, Junhao; Duan, Xinhao; Qiu, Jingfu] Chongqing Med Univ, Sch Publ Hlth & Management, Dept Hlth Lab Technol, Chongqing 400016, Peoples R China; [Zou, Zhen] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China; [Zou, Zhen; Chen, Chengzhi] Chongqing Med Univ, Dongsheng Lung Brain Dis Joint Lab, Chongqing 400016, Peoples R China; [Qiu, Yu] Chongqing Med Univ, Affiliated Univ Town Hosp, Dept Neurol, Chongqing 401331, Peoples R China; [Sumayyah, Golamaully] Chongqing Med Univ, Affiliated Hosp 1, Dept Pharm, Chongqing 400016, Peoples R China; [Jiang, Xuejun] Chongqing Med Univ, Ctr Expt Teaching Publ Hlth, Expt Teaching & Management Ctr, Chongqing 400016, Peoples R China; [Chen, Chengzhi] Chongqing Med Univ, Sch Publ Hlth & Management, Dept Occupat & Environm Hlth, Chongqing 400016, Peoples R China"

通信作者："Chen, CZ; Qiu, JF (通讯作者)，1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：PHYTOMEDICINE

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000792208600003

JCR分区：Q1

影响因子：7.9

年份：2022

卷号：99

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Huoxiangzhengqi; Lipopolysaccharide; Inflammation; Gut microbiota

摘要："Background: Huoxiangzhengqi oral liquid (HX), a pharmaceutical product made from traditional Chinese med-icine formulas, has been commonly used in household medication for gastrointestinal disorders, but the mode of action remains largely unclear.Purpose: This study aims to investigate whether pretreatment with HX prevents lipopolysaccharide (LPS)-induced adverse effects and the potential mechanisms involved. Methods: Seven-week-old male C57BL/6J mice were orally administered low (1.3 ml/kg) and high doses (2.6 ml/ kg) of HX for 7 days, and subsequently subjected to a single dose of LPS at 6 mg/kg. Dexamethasone served as the positive control. Each group had ten animals.Results: The data demonstrated that either a low or high dose of HX significantly reduced the levels of inflam-mation induced by LPS in both small intestinal and cortical tissues. LPS profoundly decreased the richness and evenness of the microbiota and disrupted the composition of the intestinal microbial community, but pretreat-ment with HX did not successfully prevent dysbiosis. No significant improvements in HX against LPS were observed in intestinal local immunity or the secretion of partial gut-brain peptides. In addition, pretreatment with HX prevented the alterations in the expression levels of proteins related to the NF-Kappa B pathway, including phospho-p38, p38, phospho-p44/42, p44/42, p50 and p65 induced by LPS. Conclusion: Herein, we demonstrated for the first time that the preventive effects of HX against LPS mainly occur through the inhibition of inflammation. These findings provide novel evidence that HX may serve as a new agent for the prevention of gastrointestinal inflammation-related disorders."

基金机构："key project of Natural Science Foundation of Chongqing [cstc2020jcyj-zdxmX0029]; Science and Technology Research Program of Chongqing Municipal Education Commission [KJQN201900418, KJQN201900419]; Young talent of Bayu scholar"

基金资助正文：This study was supported by key project of Natural Science Foundation of Chongqing (cstc2020jcyj-zdxmX0029); Science and Technology Research Program of Chongqing Municipal Education Commission (KJQN201900418 and KJQN201900419). Both Zhen Zou and Chengzhi Chen were supported by the Young talent of Bayu scholar.