LncRNA136131 suppresses apoptosis of renal tubular epithelial cells in acute kidney injury by targeting the miR-378a-3p/Rab10 axis

作者全名："Wu, Zhifen; Pan, Jian; Yang, Jurong; Zhang, Dongshan"

作者地址："[Wu, Zhifen; Yang, Jurong] Chongqing Med Univ, Affiliated Hosp 3, Dept Nephrol, Chongqing, Peoples R China; [Wu, Zhifen; Pan, Jian; Zhang, Dongshan] Cent South Univ, Xiangya Hosp 2, Dept Emergency Med, Changsha, Hunan, Peoples R China; [Wu, Zhifen; Pan, Jian; Zhang, Dongshan] Cent South Univ, Xiangya Hosp 2, Emergency Med & Difficult Dis Inst, Changsha, Hunan, Peoples R China"

通信作者："Yang, JR (通讯作者)，Chongqing Med Univ, Affiliated Hosp 3, Dept Nephrol, Chongqing, Peoples R China.; Zhang, DS (通讯作者)，Cent South Univ, Xiangya Hosp 2, Dept Emergency Med, Changsha, Hunan, Peoples R China.; Zhang, DS (通讯作者)，Cent South Univ, Xiangya Hosp 2, Emergency Med & Difficult Dis Inst, Changsha, Hunan, Peoples R China."

来源：AGING-US

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000793980700020

JCR分区：Q2

影响因子：5.2

年份：2022

卷号：14

期号：8

开始页：3666

结束页：3686

文献类型：Article

关键词：AKI; lncRNA; miR-378a-3p; Rab10; apoptosis

摘要："The pathogenesis of acute kidney injury (AKI) is not fully understood. To date, the exact role and regulatory mechanism of long non-coding RNA (lncRNA)136131 in AKI remains unclear. Here, we demonstrate that lncRNA136131 in BUMPT cells is induced by antimycin A. Furthermore, after incubating BUMPT cells in antimycin for two hours, lncRNA136131 prevented BUMPT cell apoptosis and cleaved caspase-3 expression. Mechanistically, lncRNA136131 sponged miR-378a-3p and then increased the expression of Rab10 to suppress apoptosis. Finally, I/R-induced decline of renal function, tubular damage, renal tubular cells apoptosis, and the upregulation of cleaved caspase-3 were aggravated by lncRNA136131 siRNA. In contrast, this effect was attenuated by the overexpression of lncRNA136131. In conclusion, lncRNA136131 protected against I/R-induced AKI progression by targeting miR-378a-3p/Rab10 and may be utilized as a novel target for AKI therapeutics."

基金机构："National Natural Science Foundation of China [81870475]; Basic and Frontier Research Program of Chongqing [cstc2017jcyjBX0014]; Changsha Science and Technology Bureau Project [kq2001039]; Key Project of Hunan Provincial Science and Technology Innovation [2020SK1014]; Department of Science and Technology of Hunan Province Project of International Cooperation and Exchanges [2020WK2009]; Fundamental Research Funds for the Central Universities of Central South University [2020zzts282]; Scientific Research Incubation Project of the Third Affiliated Hospital of Chongqing Medical University [KY08031, KY20078]"

基金资助正文："The study was supported by grants from the National Natural Science Foundation of China [81870475] , Basic and Frontier Research Program of Chongqing [cstc2017jcyjBX0014] , Changsha Science and Technology Bureau Project [kq2001039] , Key Project of Hunan Provincial Science and Technology Innovation [2020SK1014] , Department of Science and Technology of Hunan Province Project of International Cooperation and Exchanges [2020WK2009] , and Fundamental Research Funds for the Central Universities of Central South University [2020zzts282] , Scientific Research Incubation Project of the Third Affiliated Hospital of Chongqing Medical University (KY20078) , Scientific Research Incubation Project of the Third Affiliated Hospital of Chongqing Medical University (KY08031) ."