Allantoin-functionalized silk fibroin/sodium alginate transparent scaffold for cutaneous wound healing

作者全名："Xie, Haojiang; Bai, Qiao; Kong, Fankai; Li, Yang; Zha, Xiaoying; Zhang, Lingqin; Zhao, Yiming; Gao, Shasha; Li, Ping; Jiang, Qifeng"

作者地址："[Xie, Haojiang; Kong, Fankai; Li, Yang; Zha, Xiaoying; Zhang, Lingqin; Zhao, Yiming; Gao, Shasha; Li, Ping; Jiang, Qifeng] Chongqing Med Univ, Med Informat Coll, Chongqing 400016, Peoples R China; [Bai, Qiao] Southwest Hosp, Dept Pathol, Chongqing 400038, Peoples R China"

通信作者："Jiang, QF (通讯作者)，Chongqing Med Univ, Med Informat Coll, Chongqing 400016, Peoples R China."

来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000797064200004

JCR分区：Q1

影响因子：8.2

年份：2022

卷号：207

期号：　

开始页：859

结束页：872

文献类型：Article

关键词：Silk fibroin; Sodium alginate; Cutaneous wound healing; Allantoin

摘要："In clinical application, it's highly desirable for developing bio-functionalized cutaneous scaffold with transparent features for convenient observation, excellent biocompatibility, and high efficiency for promoting wound repair. Herein, allantoin-functionalized composite hydrogel was developed by coupling silk fibroin (SF) and sodium alginate (SA) for treatment of cutaneous wounds. The prepared allantoin-functionalized SF-SA composite scaffolds (AFAS) exhibited excellent mechanical properties, especially featured by similar ultimate tensile strength (UTS) and elongation at breaking to human skin. Besides, the solvent-casting method guaranteed the AFAS to obtain highly transparent properties with sufficient moisture permeability and excellent adhesion in wet state. In vitro cellular experiments demonstrated excellent biocompatibility of the scaffold that attachment and proliferation of NIH-3T3 fibroblast cells was promoted in the presence of AFAS. Furthermore, the scaffolds exhibited efficient hemostatic property, based on rat hepatic hemorrhage model. In a cutaneous excisional mouse wound model, the AFAS significantly improved the wound closure rate, compared with pure SF-SA scaffolds and blank control. Moreover, the histomorphological assessments showed that AFAS facilitated the integrity of skin and wound healing process by enhancing collagen deposition, re-epithelialization and vascularization at wound site. The results demonstrate that the novel allantoin-functionalized SF/SA transparent hydrogel has great potential for clinical treatment of cutaneous wound."

基金机构：Chongqing Research Program of Basic Research and Frontier Technology [cstc2019jcyjmsxmX0614]; Science and Technology Research Program of Chongqing Municipal Education Commission [KJQN201900414]

基金资助正文："Acknowledgments This research was supported by Chongqing Research Program of Basic Research and Frontier Technology (Program No. cstc2019jcyjmsxmX0614) , Science and Technology Research Program of Chongqing Municipal Education Commission (Program No. KJQN201900414) ."