Exploring Key Genes and Mechanisms in Respiratory Syncytial Virus-Infected BALB/c Mice via Multi-Organ Expression Profiles

作者全名："He, Yu; Wang, Zhili; Wei, Jianhua; Yang, Zhongying; Ren, Luo; Deng, Yu; Chen, Shiyi; Zang, Na; Liu, Enmei"

作者地址："[He, Yu; Wang, Zhili; Wei, Jianhua; Yang, Zhongying; Ren, Luo; Deng, Yu; Chen, Shiyi; Zang, Na; Liu, Enmei] Chongqing Med Univ, Minist Educ Key Lab Child Dev & Disorders, Chongqing Key Lab Pediat, Natl Clin Res Ctr Child Hlth & Disorders,Dept Resp, Chongqing, Peoples R China"

通信作者："Zang, N; Liu, EM (通讯作者)，Chongqing Med Univ, Minist Educ Key Lab Child Dev & Disorders, Chongqing Key Lab Pediat, Natl Clin Res Ctr Child Hlth & Disorders,Dept Resp, Chongqing, Peoples R China."

来源：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000797335300001

JCR分区：Q2

影响因子：5.7

年份：2022

卷号：12

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：respiratory syncytial virus (RSV); multi-organ; hemopexin (Hpx); platelet degranulation; neutrophil degranulation

摘要："Respiratory syncytial virus (RSV) a leading cause of pediatric and adult morbidity and mortality worldwide. It can cause complications in multiple organs, thus increasing hospital stays and costs. However, RSV-based studies have primarily focused on effects in the lungs and blood, thereby potentially neglecting critical genes and pathways. Hence, studying RSV infection via a novel multi-organ approach is important. In this study, lung, intestine, brain, and spleen tissues from six BALB/c mice (6-8 weeks old; three in control group and three in RSV-infected group) were subjected to RNA sequencing. Differentially expressed genes (DEGs) in each organ were obtained and functional enrichment analysis was performed. We first used CIBERSORT to evaluate the immune-infiltration landscape. Subsequently, common DEGs (co-DEGs) among the four organs were analyzed to identify key genes and pathways. After quantitative reverse transcription-polymerase chain reaction, western blotting, and external validation analysis of key hub genes, their correlation with immune cells and potential functions were explored. We found that the host response to RSV infection varied among the four organs regarding gene expression profiles and immune cell infiltration. Analysis of the 16 co-DEGs indicated enrichment in the platelet and neutrophil degranulation pathways. Importantly, the key gene hemopexin (Hpx) was strongly correlated with the immune cell fraction in the lungs and may participate in the regulation of platelet activation and immune response."

基金机构：National Natural Science Foundation of China [82070013]; Chongqing Yuzhong District Science and Technology Bureau Project [[2020]27]; Chongqing Postgraduate Research Innovation Project [CYB20155]

基金资助正文："We thank Professor Yupeng Cun from the Children's Hospital of Chongqing Medical University for methodological guidance on this paper. This study was supported by the National Natural Science Foundation of China (82070013), Chongqing Yuzhong District Science and Technology Bureau Project ([2020]27), and Chongqing Postgraduate Research Innovation Project (CYB20155)."