Anticonvulsant Effect of Carbenoxolone on Chronic Epileptic Rats and Its Mechanism Related to Connexin and High-Frequency Oscillations

作者全名："Liu, Benke; Ran, Xiao; Yi, Yanjun; Zhang, Xinyu; Chen, Hengsheng; Hu, Yue"

作者地址："[Liu, Benke; Ran, Xiao; Yi, Yanjun; Zhang, Xinyu; Chen, Hengsheng; Hu, Yue] Chongqing Med Univ, Dept Neurol, Childrens Hosp, Chongqing, Peoples R China; [Liu, Benke; Ran, Xiao; Yi, Yanjun; Zhang, Xinyu; Chen, Hengsheng; Hu, Yue] Minist Educ, Natl Clin Res Ctr Child Hlth & Disorders, Chongqing Key Lab Pediat, China Int Sci & Technol Cooperat Base Child Dev &, Chongqing, Peoples R China; [Liu, Benke] Jinan Univ, Shenzhen Baoan Womens & Childrens Hosp, Shenzhen, Peoples R China"

通信作者："Hu, Y (通讯作者)，Chongqing Med Univ, Dept Neurol, Childrens Hosp, Chongqing, Peoples R China.; Hu, Y (通讯作者)，Minist Educ, Natl Clin Res Ctr Child Hlth & Disorders, Chongqing Key Lab Pediat, China Int Sci & Technol Cooperat Base Child Dev &, Chongqing, Peoples R China."

来源：FRONTIERS IN MOLECULAR NEUROSCIENCE

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000798942300001

JCR分区：Q2

影响因子：4.8

年份：2022

卷号：15

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：carbenoxolone; chronic epilepsy; connexin; ripples; fast ripples

摘要："ObjectiveThis study was designed to investigate the influence and mechanism of gap junction carbenoxolone (CBX) on dynamic changes in the spectral power of ripples and fast ripples (FRs) in the hippocampus of chronic epileptic rats. MethodsThe lithium-pilocarpine (PILO) status epilepticus (SE) model (PILO group) and the CBX pretreatment model (CBX + PILO group) were established to analyze dynamic changes in the spectral power of ripples and FRs, and the dynamic expression of connexin (CX)26, CX32, CX36, and CX43 in the hippocampus of chronic epileptic rats. ResultsWithin 28 days after SE, the number of spontaneous recurrent seizures (SRSs) in the PILO group was significantly higher than that in the CBX + PILO group. The average spectral power of FRs in the PILO group was significantly higher than the baseline level at 1 and 7 days after SE. The average spectral power of FRs in the PILO group was significantly higher than that in the CBX + PILO group at 1, 7, and 14 days after SE. Seizures induced an increase in CX43 expression at 1 and 7 days after SE, but had no significant effect on CX26, CX36, or CX32. CBX pretreatment did not affect the expression of CXs in the hippocampus of normal rats, but it inhibited the expression of CX43 in epileptic rats. The number of SRSs at 2 and 4 weeks after SE had the highest correlation with the average spectral power of FRs; the average spectral power of FRs was moderately correlated with the expression of CX43. ConclusionThe results of this study indicate that the energy of FRs may be regulated by its interference with the expression of CX43, and thus, affect seizures. Blocking the expression of CX43 thereby reduces the formation of pathological high-frequency oscillations (HFOs), making it a promising strategy for the treatment of chronic epilepsy."

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