Ovarian inflammatory mRNA profiles of a dehydroepiandrosterone plus high-fat diet-induced polycystic ovary syndrome mouse model

作者全名："Ullah, Amin; Wang, Mei-Jiao; Yang, Jun-Pu; Adu-Gyamfi, Enoch Appiah; Czika, Armin; Sah, Sanjay Kumar; Feng, Qian; Wang, Ying-Xiong"

作者地址："[Ullah, Amin; Wang, Mei-Jiao; Yang, Jun-Pu; Adu-Gyamfi, Enoch Appiah; Czika, Armin; Sah, Sanjay Kumar; Wang, Ying-Xiong] Chongqing Med Univ Chongqing, Joint Int Res Lab Reprod & Dev, Chongqing, Peoples R China; [Ullah, Amin; Adu-Gyamfi, Enoch Appiah; Czika, Armin; Sah, Sanjay Kumar] Chongqing Med Univ Chongqing, Sch Publ Hlth, Dept Reprod Sci, Chongqing, Peoples R China; [Wang, Mei-Jiao; Yang, Jun-Pu; Wang, Ying-Xiong] Chongqing Med Univ Chongqing, Sch Basic Med, Dept Physiol, Chongqing, Peoples R China; [Feng, Qian] Tradit Chinese Med Chongqing, Chongqing Hosp, Chongqing, Peoples R China"

通信作者："Wang, YX (通讯作者)，Chongqing Med Univ Chongqing, Joint Int Res Lab Reprod & Dev, Chongqing, Peoples R China.; Wang, YX (通讯作者)，Chongqing Med Univ Chongqing, Sch Basic Med, Dept Physiol, Chongqing, Peoples R China."

来源：REPRODUCTIVE BIOMEDICINE ONLINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000798982100003

JCR分区：Q1

影响因子：4

年份：2022

卷号：44

期号：5

开始页：791

结束页：802

文献类型：Article

关键词：Gene expression; Inflammation; Polycystic ovary syndrome; RNA sequencing

摘要："Research question: What is the expression pattern of inflammatory mRNA profiles of a dehydroepiandrosterone (DHEA) plus high-fat diet (HFD)-induced polycystic ovary syndrome (PCOS) mouse model? Design: RNA sequencing was performed to investigate the mRNA expression profiles in the ovarian tissues of a DHEA plus HFD-induced PCOS mouse model. Six samples were divided into two groups (control and PCOS), with three biological replicates in each group. This was followed by hierarchical clustering, gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses. The relative expression levels of nine inflammatory genes were validated via quantitative reverse-transcription polymerase chain reaction. Results: A total of 436 genes were differentially expressed between the control and PCOS mice. Out of these, 137 genes were up-regulated while 299 genes were down-regulated. Gene ontology analysis indicated that differentially expressed mRNA were associated with T cell-mediated cytotoxicity and homocysteine metabolic processes. Pathway analysis further showed that these abnormally expressed mRNA were associated with signalling pathways, such as NF-kB signalling, tyrosine metabolism and phenylalanine metabolism. All these pathways are involved in chronic inflammation and PCOS. Conclusion: The differentially expressed genes are potentially involved in the inflammation that is evident in PCOS, and so could serve as therapeutic options against the disease. Nevertheless, prospective studies are needed to test this hypothesis."

基金机构："Chongqing Natural Science Foundation [cstc2020jcyj-msxmX0294, cstc2018jxj1130065, jxyn2019-2-7]; Science and Technology Project of Chongqing Yuzhong District [20200103]; National Natural Science Foundation of China [82171624, 82104923]; Scientific Research and Innovation Experiment Project of Chongqing Medical University [SRIEP202002, SRIEP202106]; Chongqing Municipal Health Bureau Chinese Medicine Technology Project [ZY20182044]"

基金资助正文："This study was supported by grants from the Chongqing Natural Science Foundation (no. cstc2020jcyj-msxmX0294, no. cstc2018jxj1130065, no. jxyn2019-2-7) , the Science and Technology Project of Chongqing Yuzhong District (no. 20200103) , the National Natural Science Foundation of China (no. 82171624, no. 82104923) , the Scientific Research and Innovation Experiment Project of Chongqing Medical University (SRIEP202002, SRIEP202106) , and the Chongqing Municipal Health Bureau Chinese Medicine Technology Project (no. ZY20182044) ."