Neutrophil depletion reduces interstitial cajal-like cell injury and alleviates inflammation-induced motor dysfunction in guinea-pig gallbladder during acute cholecystitis
作者全名:"Zhang, Li; Yang, Bin; Xiao, Yong; Zhang, Bingqiang; Yu, Baoping; Kuang, Youlin"
作者地址:"[Zhang, Li; Zhang, Bingqiang] Chongqing Med Univ, Affiliated Hosp 1, Dept Gastroenterol, 1 Youyi Rd, Chongqing 400016, Peoples R China; [Yang, Bin; Xiao, Yong; Yu, Baoping] Wuhan Univ, Renmin Hosp, Dept Gastroenterol, 238 Jiefang Rd, Wuhan 430060, Hubei, Peoples R China; [Kuang, Youlin] Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, 1 Youyi Rd, Chongqing 400016, Peoples R China"
通信作者:"Kuang, YL (通讯作者),Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, 1 Youyi Rd, Chongqing 400016, Peoples R China."
来源:IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
ESI学科分类:PHARMACOLOGY & TOXICOLOGY
WOS号:WOS:000799192400002
JCR分区:Q3
影响因子:2.2
年份:2022
卷号:25
期号:4
开始页:435
结束页:441
文献类型:Article
关键词:Common bile duct ligation; Gallbladder dysmotility; Guinea pigs; Interstitial cajal-like cells; Neutrophils; Ultrastructure features
摘要:"Objective(s): Gallbladder interstitial Cajal-like cells (ICLCs) are known as some of the players in the complex motility mechanisms affecting gallbladder motility. This study aims to explore the mechanism of guinea-pig gallbladder motility disorders during Acute Cholecystitis (AC), focusing on the relationships between neutrophil alterations, gallbladder ICLCs, and smooth muscle contractility. Materials and Methods: Forty-eight guinea pigs were randomly divided into four groups: normal, sham, common bile duct ligation (CBDL), and anti-PMN (anti-polymorphonuclear antibody treated +CBDL). Hematoxylin and eosin-stained slides from each gallbladder sample were examined for inflammation, and myeloperoxidase (MPO) activity was evaluated. The contractile response of gallbladder muscle to Ach, CCK-8, and KCl was registered by a tension transducer, and ultrastructure features of ICLCs were observed. Results: Pretreatment with anti-PMN significantly reduced the circulating neutrophils by 80% and also considerably decreased the gallbladder MPO activity by 52.9% compared with the CBDL group (P<0.05). After adding Ach, CCK-8, and KCl, the contraction ability in CBDL and anti-PMN groups was lower than those of normal and sham groups (P<0.05), and they were increased substantially in the anti-PMN group compared with the CBDL group (P<0.05). Transmission electron microscopy confirmed that the cytoplasm of the neutrophils was full of granules, and neutrophils contacted closely with ICLCs. The ultrastructure of ICLCs in the anti-PMN group was less inflamed and the endoplasmic reticulum was mildly dilated, and cell processes also increased. Conclusion: Anti-PMN could relieve the ultrastructure injury of ICLCs and alleviate gallbladder dysmotility during AC. Neutrophils may damage gallbladder ICLCs at first followed by dysmotility."
基金机构:National Natural Science Foundation of China (NSFC) [81170351]
基金资助正文:The results presented in this paper were part of a student thesis. This study was supported by the National Natural Science Foundation of China (NSFC) (No. 81170351) .