TiO2@Ag nanozyme enhanced electrochemiluminescent biosensor coupled with DNA nanoframework-carried emitters and enzyme-assisted target recycling amplification for ultrasensitive detection of microRNA

作者全名："Shen, Bo; Wu, Qian; Fan, Yunpeng; Wu, Haiping; Li, Xinmin; Zhao, Xiaofen; Wang, Yuwei; Ding, Shijia; Zhang, Juan"

作者地址："[Shen, Bo; Fan, Yunpeng; Li, Xinmin; Zhao, Xiaofen; Wang, Yuwei; Zhang, Juan] Chongqing Hosp Tradit Chinese Med, Chongqing Key Lab Sichuan Chongqing Coconstruct Di, Chongqing 400021, Peoples R China; [Shen, Bo; Fan, Yunpeng; Li, Xinmin; Zhao, Xiaofen; Wang, Yuwei; Zhang, Juan] Chongqing Hosp Tradit Chinese Med, Dept Lab Med, Chongqing 400021, Peoples R China; [Wu, Qian] Chongqing Med Univ, Dept Lab Med, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Wu, Haiping; Ding, Shijia] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Zhang, J (通讯作者)，Chongqing Hosp Tradit Chinese Med, Chongqing Key Lab Sichuan Chongqing Coconstruct Di, Chongqing 400021, Peoples R China.; Zhang, J (通讯作者)，Chongqing Hosp Tradit Chinese Med, Dept Lab Med, Chongqing 400021, Peoples R China.; Ding, SJ (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China."

来源：CHEMICAL ENGINEERING JOURNAL

ESI学科分类：ENGINEERING

WOS号：WOS:000803676000003

JCR分区：Q1

影响因子：15.1

年份：2022

卷号：445

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：MicroRNA analysis; DNA nanoframework; Nanozymes; Target recycling amplification; Electrochemiluminescent biosensor

摘要："MicroRNAs (miRNAs) have been discovered as promising biomarkers for cancer early diagnosis. Constructing sensitive biosensing strategy for detecting extremely low-abundance miRNAs in biological samples is urgently required. Herein, an efficient electrochemiluminescent (ECL) biosensor was developed for ultrasensitive detec-tion of miRNA coupled with DNA nanoframework-carried emitters (DNF-Dox-ABEI) and T7 exonuclease-assisted target recycling amplification (T7 exo-TRA). The fabricated DNF-Dox-ABEI emitters could load numerous luminophore molecules, and silver nanoparticles-coated TiO2 (TiO2@Ag NPs) nanozymes held excellent peroxidase-like activity in catalyzing H2O2 to produce enormous reactive oxygen species (ROS), which further reacted with ABEI to enhance ECL emission. Meanwhile, the T7 exo-TRA could effectively convert target hy-bridization event into amplifying signals for further improving sensitivity. Consequently, the constructed ECL biosensor enabled ultrasensitive detection of miRNA-155 with a broad linear range from 1.0 fM to 500.0 pM and a low detection limit of 0.45 fM (S/N = 3). Furthermore, it was noted that the biosensing strategy successfully achieved miRNA-155 analysis from breast cancer cells. Therefore, the constructed biosensor in the present study provided a potential tool in cancer early diagnosis and a novel approach for designing efficient ECL nanomaterials."

基金机构："National Natural Science Founda-tion of China [81873980]; Natural Science Foundation of Chongq-ing, China [cstc2021jcyj-msxmX0325]; Incentive Plan for Scientific Institution of Chongqing, China [cstc2021jxjl130038]"

基金资助正文："Acknowledgements This study was sponsored by the National Natural Science Founda-tion of China (81873980) , the Natural Science Foundation of Chongq-ing, China (cstc2021jcyj-msxmX0325) , and the Incentive Plan for Scientific Institution of Chongqing, China (cstc2021jxjl130038) ."