Astragaloside IV Ameliorates Isoprenaline-Induced Cardiac Fibrosis in Mice via Modulating Gut Microbiota and Fecal Metabolites

作者全名："Du, Xu-Qin; Shi, Li-Peng; Chen, Zhi-Wei; Hu, Jin-Yuan; Zuo, Biao; Xiong, Yu; Cao, Wen-Fu"

作者地址："[Du, Xu-Qin; Shi, Li-Peng; Chen, Zhi-Wei; Hu, Jin-Yuan; Zuo, Biao; Xiong, Yu; Cao, Wen-Fu] Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing, Peoples R China; [Du, Xu-Qin; Shi, Li-Peng; Chen, Zhi-Wei; Hu, Jin-Yuan; Zuo, Biao; Xiong, Yu; Cao, Wen-Fu] Chongqing Med Univ, Chongqing Key Lab Tradit Chinese Med Prevent & Cur, Chongqing, Peoples R China; [Cao, Wen-Fu] Chongqing Med Univ, Dept Chinese Tradit Med, Affiliated Hosp 1, Chongqing, Peoples R China"

通信作者："Cao, WF (通讯作者)，Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing, Peoples R China.; Cao, WF (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Tradit Chinese Med Prevent & Cur, Chongqing, Peoples R China.; Cao, WF (通讯作者)，Chongqing Med Univ, Dept Chinese Tradit Med, Affiliated Hosp 1, Chongqing, Peoples R China."

来源：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000804111100001

JCR分区：Q2

影响因子：5.7

年份：2022

卷号：12

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Astragaloside IV; cardiac fibrosis; gut microbiota; fecal metabolites; Akkermansia

摘要："AimGut microbiota is of crucial importance to cardiac health. Astragaloside IV (AS-IV) is a main active ingredient of Huangqi, a traditional edible and medicinal herb that has been shown to have beneficial effects on cardiac fibrosis (CF). However, it is still uncertain whether the consumption of AS-IV alleviates cardiac fibrosis through the gut microbiota and its metabolites. Therefore, we assessed whether the anti-fibrosis effect of AS-IV is associated with changes in intestinal microbiota and fecal metabolites and if so, whether some specific gut microbes are conducive to the benefits of AS-IV. MethodsMale C57BL-6J mice were subcutaneously injected with isoprenaline (ISO) to induce cardiac fibrosis. AS-IV was administered to mice by gavage for 14 days. The effects of AS-IV on cardiac function, myocardial enzyme, cardiac weight index (CWI), and histopathology of ISO-induced CF mice were investigated. Moreover, 16S rRNA sequencing was used to establish gut-microbiota profiles. Fecal-metabolites profiles were established using the liquid chromatograph-mass spectrometry (LC-MS). ResultsAS-IV treatment prevented cardiac dysfunction, ameliorated myocardial damage, histopathological changes, and cardiac fibrosis induced by ISO. AS-IV consumption increased the richness of Akkermansia, Defluviitaleaceae_UCG-011, and Rikenella. AS-IV also modulated gut metabolites in their feces. Among 141 altered gut metabolites, amino acid production was sharply changed. Furthermore, noticeable correlations were found between several specific gut microbes and altered fecal metabolites. ConclusionsAn increase of Akkermansia, Defluviitaleaceae_UCG-011, and Rikenella abundance, and modulation of amino acid metabolism, may contribute to the anti-fibrosis and cardiac protective effects of Astragaloside IV."

基金机构：Chongqing Medical University Postdoctoral Foundation [R11004]; Chongqing Postdoctoral Science Foundation [cstc2021jcyj-bshX0215]; Xinglin program of Chongqing TCM/TCM-integrated Key discipline [2021-ZDXK-bshx03]

基金资助正文："Funding This work was supported by Chongqing Medical University Postdoctoral Foundation (No. R11004), Chongqing Postdoctoral Science Foundation (No. cstc2021jcyj-bshX0215), and Xinglin program of Chongqing TCM/TCM-integrated Key discipline (No. 2021-ZDXK-bshx03, No.2021-ZDXK-DB03)."