Highly sensitive fluorescence multiplexed miRNAs biosensors for accurate clinically diagnosis lung cancer disease using LNA-modified DNA probe and DSN enzyme

作者全名："Djebbi, Khouloud; Xing, Juanxia; Weng, Ting; Bahri, Mohamed; Elaguech, Mohamed Amin; Du, Chao; Shi, Biao; Hu, Li; He, Shixuan; Liao, Pu; Tlili, Chaker; Wang, Deqiang"

作者地址："[Djebbi, Khouloud; Weng, Ting; Bahri, Mohamed; Elaguech, Mohamed Amin; Shi, Biao; He, Shixuan; Tlili, Chaker; Wang, Deqiang] Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China; [Djebbi, Khouloud; Weng, Ting; Bahri, Mohamed; Elaguech, Mohamed Amin; Shi, Biao; He, Shixuan; Liao, Pu; Tlili, Chaker; Wang, Deqiang] Univ Chinese Acad Sci UCAS, Chongqing Sch, Chongqing 400714, Peoples R China; [Djebbi, Khouloud; Bahri, Mohamed; Elaguech, Mohamed Amin; Wang, Deqiang] Univ Chinese Acad Sci UCAS, Beijing 100049, Peoples R China; [Xing, Juanxia; Du, Chao; Hu, Li; Liao, Pu] Chongqing Gen Hosp, Dept Lab Med, Chongqing 400014, Peoples R China; [Xing, Juanxia; Du, Chao; Liao, Pu] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Tlili, C; Wang, D (通讯作者)，Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China.; Liao, P (通讯作者)，Univ Chinese Acad Sci UCAS, Chongqing Sch, Chongqing 400714, Peoples R China."

来源：ANALYTICA CHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:000804193000011

JCR分区：Q1

影响因子：6.2

年份：2022

卷号：1208

期号：　

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结束页：　

文献类型：Article

关键词：Lung cancer; microRNAs; Fluorescence assay; Multiplexed; DSN; Magnetic beads

摘要："With the emergence of microRNAs as key biomarkers for disease diagnosis such as lung cancer, various techniques have been settled for their detection. However, these current methods require different amplification steps since numerous challenges for detecting circulating miRNAs are attributable to their intrinsic properties accounting for tiny sizes, high sequence similarity, and low abundance. Duplex specific nuclease (DSN)-based microRNA amplification has recently gained interest in biosensing applications thanks to its catalytic activity based on target recycling. In this context, we designed a highly selective, sensitive, and multiplexed fluorescence-based biosensor combining DSN enzyme and magnetic & nbsp;beads to detect three distinct microRNAs, including microRNA-21, microRNA-210, and microRNA-486-5p. By exploiting the above approach, we were able to detect as low as 98 aM, 120 aM, and 300 aM of mir-21, miR-210, and miR-486-5p, respectively. Furthermore, this recommended strategy displays a high selectivity toward an entirely matched target than the off-target. These results are ascribed to the potent DSN enzyme activity and to the locked nucleic acid (LNA)-modified DNA probe that boosted the heteroduplex probe/target stability. Lastly, our proposed method was applied to detect microRNAs in the serum samples and displayed a high efficacy to discriminate between healthy controls and lung cancer patients. Furthermore, the analytical accuracy of the proposed strategy was validated with the computed tomography (CT) technique of the chest. Thus based on these findings, this strategy could open new directions for detecting microRNAs associated with several diseases. (c) 2022 Elsevier B.V. All rights reserved."

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