HTR1D functions as a key target of HOXA10-AS/miR-340-3p axis to promote the malignant outcome of pancreatic cancer via PI3K-AKT signaling pathway

作者全名："Wu, Wu; Li, Qujin; Zhu, Zhu; Li, Chunming; Lu, Peilin; Zhou, Xi; Huang, Yujing; Liu, Yan; Wang, Menghao; Gong, Jianping"

作者地址："[Wu, Wu; Li, Qujin; Zhu, Zhu; Li, Chunming; Liu, Yan; Wang, Menghao; Gong, Jianping] Chongqing Med Univ, Dept Hepatobiliary Surg, Affiliated Hosp 2, Chongqing, Peoples R China; [Lu, Peilin; Huang, Yujing] Chongqing Med Univ, Dept Dermatol, Affiliated Hosp 1, Chongqing, Peoples R China; [Zhou, Xi] Southwest Med Univ, Dept Gastroenterol, Affiliated Hosp, Luzhou, Sichuan, Peoples R China; [Liu, Yan] Fifth Peoples Hosp Chengdu, Dept Gastroenterol, Chengdu, Sichuan, Peoples R China"

通信作者："Liu, Y; Wang, MH; Gong, JP (通讯作者)，Chongqing Med Univ, Dept Hepatobiliary Surg, Affiliated Hosp 2, Chongqing, Peoples R China.; Liu, Y (通讯作者)，Fifth Peoples Hosp Chengdu, Dept Gastroenterol, Chengdu, Sichuan, Peoples R China."

来源：INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000804489600001

JCR分区：Q1

影响因子：9.2

年份：2022

卷号：18

期号：9

开始页：3777

结束页：3794

文献类型：Article

关键词：pancreatic cancer; ceRNA; HTR1D; HOXA10-AS; miR-340-3p

摘要："Competing endogenous RNAs (ceRNAs) are a newly discovered class of molecular regulators involved in many diseases, especially tumors. Therefore, exploration of the potential ceRNA regulatory network regarding the occurrence and development of pancreatic cancer will provide a new theoretical basis for its diagnosis and treatment. Based on the above background, we applied a bioinformatics approach to mine the public database The Cancer Genome Atlas (TCGA) and performed a series of subsequent molecular biology assays to confirm the hypothesis that HOXA10-AS/ miR-340-3p/HTR1D axis could modulate the malignant progression of pancreatic cancer. Here, our present study demonstrated that the expression level of HTR1D, positively correlated with the level of lncRNA HOXA10-AS and negatively associated with the level of miR-340-3p, was significantly increased in pancreatic cancer cell lines (PCs) compared with that in normal HPDE6-C7 cells. Knocking down HTR1D obviously inhibited the proliferation and migration of PCs and promoted apoptosis by upregulating p-AKT. Elevated miR-340-3p blocked the progression of pancreatic cancer by downregulating HTR1D. Lessened level of lncRNA HOXA10-AS reduced the sponging of miR-340-3p, resulting in an increase of miR-340-3p and a subsequent decrease of HTR1D to ultimately suppress the malignant biological behaviors of cancer. These data illustrated that the HOXA10-AS/miR-340-3p/HTR1D ceRNA axis acted a crucial part in the malignant biological behavior of pancreatic cancer in an AKT-dependent manner."

基金机构："National Natural Science Foundation of China Youth Science Fund [82100604, H0307]"

基金资助正文："This research was supported by National Natural Science Foundation of China Youth Science Fund (82100604, H0307) ."