Adipose-derived stromal/stem cells are verified to be potential seed candidates for bio-root regeneration in three-dimensional culture

作者全名："Yuan, Yu; Zhang, Xiaonan; Zhan, Yuzhen; Tang, Song; Deng, Pingmeng; Wang, Zhenxiang; Li, Jie"

作者地址："[Yuan, Yu; Zhang, Xiaonan; Zhan, Yuzhen; Tang, Song; Deng, Pingmeng; Wang, Zhenxiang; Li, Jie] Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China; [Yuan, Yu; Zhang, Xiaonan; Zhan, Yuzhen; Tang, Song; Deng, Pingmeng; Wang, Zhenxiang; Li, Jie] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Yuan, Yu; Zhang, Xiaonan; Zhan, Yuzhen; Tang, Song; Deng, Pingmeng; Wang, Zhenxiang; Li, Jie] Chongqing Municipal Key Lab Oral Biomed Engn High, Chongqing, Peoples R China"

通信作者："Li, J (通讯作者)，Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China.; Li, J (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China.; Li, J (通讯作者)，Chongqing Municipal Key Lab Oral Biomed Engn High, Chongqing, Peoples R China."

来源：STEM CELL RESEARCH & THERAPY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000805834900006

JCR分区：Q1

影响因子：7.5

年份：2022

卷号：13

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Adipose-derived stromal/stem cells; Dental follicle cells; Stem cells from human exfoliated deciduous teeth; Porcine treated cellular dentin matrix; Bio-root regeneration

摘要："Background: Bio-root regeneration is a promising treatment for tooth loss. It has been reported that dental-derived stem cells are effective seed cells for bio-root construction, but further applications are limited by their few sources. Human adipose tissues have a wide range of sources and numerous studies have confirmed the ability of adipose-derived stromal/stem cells (ASCs) in regenerative medicine. In the current study, the odontogenic capacities of ASCs were compared with dental-derived stem cells including dental follicle cells (DFCs), and stem cells from human exfoliated deciduous teeth (SHEDs). Methods: The biological characteristics of ASCs, DFCs, and SHEDs were explored in vitro. Two-dimensional (2D) and three-dimensional (3D) cultures were compared in vitro. Odontogenic characteristics of porcine-treated dentin matrix (pTDM) induced cells under a 3D microenvironment in vitro were compared. The complexes (cell/pTDM) were transplanted subcutaneously into nude mice to verify regenerative potential. RNA sequencing (RNA-seq) was used to explore molecular mechanisms of different seed cells in bio-root regeneration. Results: 3D culture was more efficient in constructing bio-root complexes. ASCs exhibited good biological characteristics similar to dental-derived stem cells in vitro. Besides, pTDM induced ASCs presented odontogenic ability similar to dental-derived stem cells. Furthermore, 3D cultured ASCs/pTDM complex promoted regeneration of dentin-like, pulp-like, and periodontal fiber-like tissues in vivo. Analysis indicated that PI3K-Akt,VEGF signaling pathways may play key roles in the process of inducing ASCs odontogenic differentiation by pTDM. Conclusions: ASCs are potential seed cells for pTDM-induced bio-root regeneration, providing a basis for further research and application."

基金机构：National Natural Science Foundation of China [82071072]; Technological Research Program of Chongqing Municipal Education Commission [KJQN202000417]; Natural Science Foundation of Chongqing Yuzhong Distinct [20200113]; Scientific and Young and Middle-aged Senior Medical Talents studio of Chongqing [ZQNYXGDRCGZS2019004]

基金资助正文："This work was supported by the National Natural Science Foundation of China [Grant No. 82071072], Technological Research Program of Chongqing Municipal Education Commission [Grant No. KJQN202000417], Natural Science Foundation of Chongqing Yuzhong Distinct [Grant No. 20200113], and Scientific and Young and Middle-aged Senior Medical Talents studio of Chongqing [ZQNYXGDRCGZS2019004]."