Brusatol Inhibits Proliferation and Metastasis of Colorectal Cancer by Targeting and Reversing the RhoA/ROCK1 Pathway

作者全名："Lu, Rui-jin; Zhao, Guo-zhi; Jiang, Rong; He, Shuang; Xu, Hang; He, Jia-ming; Sun, Yue; Wu, Meng-na; Ran, Jian-hua; Chen, Di-long; Li, Jing"

作者地址："[Lu, Rui-jin; Jiang, Rong; He, Shuang; He, Jia-ming; Sun, Yue; Li, Jing] Chongqing Med Univ, Dept Histol & Embryol, Lab Stem Cell & Tissue Engn, Chongqing, Peoples R China; [Zhao, Guo-zhi] Chongqing Med Univ, Dept Urol, Affiliated Hosp 1, Chongqing, Peoples R China; [Xu, Hang; Wu, Meng-na; Ran, Jian-hua] Chongqing Med Univ, Coll Basic Med, Neurosci Res Ctr, Chongqing, Peoples R China; [Chen, Di-long] Chongqing Key Lab Dev & Utilizat Genuine Med Mat T, Chongqing, Peoples R China"

通信作者："Li, J (通讯作者)，Chongqing Med Univ, Dept Histol & Embryol, Lab Stem Cell & Tissue Engn, Chongqing, Peoples R China."

来源：BIOMED RESEARCH INTERNATIONAL

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000806025400001

JCR分区：Q3

影响因子：3.246

年份：2022

卷号：2022

期号：　

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结束页：　

文献类型：Article

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摘要："Brusatol (BRU) is an important compound extracted from Brucea javanica oil, whose pharmacological effects are able to induce a series of biological effects, including inhibition of tumor cell growth, anti-inflammatory, antiviral, and antitumor. Currently, there are so few studies about the brusatol effects on colorectal cancer that its anticancer mechanism has not been clearly defined. In this study, we made an in-depth investigation into the brusatol effect towards the proliferation and metastasis of colon cancer and the possible mechanism. The inhibitory effect of BRU on the proliferation of colorectal cancer cells was unveiled via CCK-8 method and colony formation assay, while the inhibitory effect of BRU on migration and invasion of colorectal cancer cells was revealed by scratch assay and transwell assay. In addition, Western blot results also revealed that BRU inhibited not only the expressions of RhoA and ROCK1 but also the protein expressions of EMT-related markers e-cadherin, N-cadherin, Vimentin, MMP2, and MMP9 in colon cancer cells. Through the xenotransplantation model, our in vivo experiment further verified the antitumor effect of BRU on colon cancer cells in vitro, and the results were consistent with the protein expression trend. In conclusion, BRU may inhibit the proliferation and metastasis of colorectal cancer by influencing EMT through RhoA/ROCK1 pathway."

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