A simple and green method for direct determination of hydrogen peroxide and hypochlorite in household disinfectants based on personal glucose meter

作者全名："Zhang, Hao; Gong, Zhao-Miao; Li, Yan; Yang, Feng-Qing"

作者地址："[Zhang, Hao; Gong, Zhao-Miao; Li, Yan] Chongqing Med & Pharmaceut Coll, Chongqing Key Lab High Act Tradit Chinese Drug De, Chongqing 401331, Peoples R China; [Zhang, Hao] Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China; [Yang, Feng-Qing] Chongqing Univ, Sch Chem & Chem Engn, Chongqing 401331, Peoples R China"

通信作者："Zhang, H (通讯作者)，Chongqing Med & Pharmaceut Coll, Chongqing Key Lab High Act Tradit Chinese Drug De, Chongqing 401331, Peoples R China.; Yang, FQ (通讯作者)，Chongqing Univ, Sch Chem & Chem Engn, Chongqing 401331, Peoples R China."

来源：ENZYME AND MICROBIAL TECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000806562700003

JCR分区：Q2

影响因子：3.4

年份：2022

卷号：155

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Hypochlorite; Hydrogen peroxide; Personal glucose meter; Acetylthiocholine iodide; Acetylcholinesterase; Green chemistry

摘要："A convenient and green method was developed using a personal glucose meter (PGM) for direct determination of hydrogen peroxide and hypochlorite in household disinfectants based on the acetylthiocholine iodide (ATCI)mediated reaction. In this method, acetylcholinesterase catalyzes the hydrolysis of ATCI to generate thiocholine iodide, which can trigger the reduction of K3[Fe(CN)6] to K4[Fe(CN)6] and generate a PGM detectable signal. When the hydrogen peroxide or hypochlorite is pre-incubated with the ATCI, the yield of thiocholine will be decreased because the iodine molecules can initiate the oxidation of thiocholine to disulphides, leading to a lower PGM readout. Thus, the hydrogen peroxide or hypochlorite can be directly quantified by PGM as simple as detecting glucose in blood. After being systematically investigated, the optimum conditions of the PGM method are as follows: 5.0 min of enzymatic reaction time, 6.0 mM final concentration of ATCI solution, and 10.0 min of reaction time between ATCI/H2O2 and thiocholine. Moreover, the recoveries of hydrogen peroxide in real sample spiked with three different concentrations (final concentrations of 5.0, 10.0, and 20.0 mM) are in the range of 96.3-108.4%. The recoveries of hypochlorite in real sample spiked with three different concentrations (final concentrations of 1.0, 5.0, and 10.0 mM) are in the range of 99.4-117.0%. These results indicate that the developed method can be employed for the detection of hydrogen peroxide and hypochlorite in household disinfectants."

基金机构："Chongqing Medical and Pharmaceutical College, China [YGZ2021301]"

基金资助正文："Acknowledgements This work was sponsored by Chongqing Medical and Pharmaceutical College, China (YGZ2021301) ."