Accurate 16S Absolute Quantification Sequencing Revealed Vaginal Microecological Composition and Dynamics During Mixed Vaginitis Treatment With Fufang FuRong Effervescent Suppository

作者全名："Li, Meng; Zeng, Zhen; Feng, Huijun; Cao, Yang; Zhang, Qiongqiong; Lv, Tao; Yang, Xingsheng; Song, Dianrong; Li, Ping; Hu, Lina; Fan, Shangrong; An, Ruifang; Zhang, Bei; Zhang, Lei; Liao, Qinping"

作者地址："[Li, Meng; Zhang, Qiongqiong] Tsinghua Univ, Sch Clin Med, Beijing, Peoples R China; [Li, Meng; Zeng, Zhen; Feng, Huijun; Cao, Yang; Zhang, Qiongqiong; Lv, Tao; Zhang, Lei; Liao, Qinping] Tsinghua Univ, Beijing Tsinghua Changgung Hosp, Sch Clin Med, Dept Obstet & Gynecol, Beijing, Peoples R China; [Yang, Xingsheng] Qilu Hosp Shandong Univ, Dept Obstet & Gynecol, Jinan, Peoples R China; [Song, Dianrong] Tianjin Univ Tradit Chinese Med, Affiliated Hosp 1, Gynecol Dept, Tianjin, Peoples R China; [Li, Ping] Nanjing Med Univ, Affiliated Obstet & Gynecol Hosp, Nanjing Matern & Child Hlth Care Hosp, Dept Obstet & Gynecol, Nanjing, Peoples R China; [Hu, Lina] Chongqing Med Univ, Hosp Affiliated 2, Dept Gynecol, Chongqing, Peoples R China; [Fan, Shangrong] Peking Univ Shenzhen Hosp, Dept Obstet & Gynecol, Shenzhen, Peoples R China; [An, Ruifang] Xi An Jiao Tong Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Xi'an, Peoples R China; [Zhang, Bei] Xuzhou Med Univ, Affiliated Xuzhou Clin Coll, Xuzhou Cent Hosp, Dept Obstet & Gynaecol, Xuzhou, Peoples R China"

通信作者："Zhang, L; Liao, QP (通讯作者)，Tsinghua Univ, Beijing Tsinghua Changgung Hosp, Sch Clin Med, Dept Obstet & Gynecol, Beijing, Peoples R China."

来源：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000806567700001

JCR分区：Q2

影响因子：5.7

年份：2022

卷号：12

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：accurate 16S absolute quantification sequencing; mixed vaginitis; microbiome; antibiotic; traditional Chinese medicine

摘要："BackgroundThe diagnosis and treatment of mixed vaginitis are more complicated than single pathogenic infections, and there may be adverse reactions and several contraindications to conventional antibiotic therapy. Therefore, this study aimed to evaluate the preliminary effects of Fufang Furong Effervescent Suppository for the management of aerobic vaginitis (AV) mixed with bacterial vaginosis (BV) using Accurate 16S absolute quantification sequencing (Accu16S). MethodsIn the present randomized, blind, multi-center clinical trial, women (20 to 55 years) who had received a diagnosis of AV+BV were randomly assigned into clindamycin positive control (n = 41) and Fufang Furong Effervescent Suppository (n = 39) groups. The follow-up occurred in three time periods (V1: -2~0 days; V2: 15-17 days; V3: 40 +/- 3 days). At each visit, two vaginal swabs, one for clinical evaluation and one for laboratory examination, were taken from each patient. The Nugent score, Donders' score, drug-related complications, recurrence rates, and microecological changes of vaginal swabs were assessed in the time three periods. ResultsAt baseline, the two groups were similar in frequency of presentation with vaginal burning, odor, abnormal discharge, and itching. No meaningful differences in Nugent and Donders' scores were detected between the two groups at stage V2 (Nugent: p = 0.67; Donders': p = 0.85) and V3 (Nugent: p = 0.97; Donders: p = 0.55). The Furong group presented fewer complications compared to the Clindamycin group. However, this difference was not statistically significant (p = 0.15). Additionally, Accu16S indicated that the total abundance of bacteria in both groups sharply decreased in stage V2, but slightly increased in V3. In stage V3, the absolute abundance of Lactobacillus in the Furong group was considerably higher compared to untreated samples (p < 0.05). On the other hand, no momentous increase was detected in the Clindamycin group (p > 0.05). ConclusionFufang Furong Effervescent Suppository can be as effective as clindamycin cream in the management of AV+BV while may restore the vagina microecosystem better."

基金机构：National Key Research and Development Program [2018YFC1707404]; Beijing Natural Science Foundation [7202239]; Beijing Traditional Chinese Medicine Science and Technology Development Fund Project [JJ-2020-09]; National Key Research and Development Program of TCM Modernization Research key project [2018YFC1707410-04]; Ministry of Science and Technology of China Biotechnology Development Center

基金资助正文："This trial was funded by National Key Research and Development Program (2018YFC1707404), Beijing Natural Science Foundation (7202239), Beijing Traditional Chinese Medicine Science and Technology Development Fund Project (JJ-2020-09), Ministry of Science and Technology of China Biotechnology Development Center, National Key Research and Development Program of TCM Modernization Research key project (2018YFC1707410-04)."