Wnt3a-induced ST2 decellularized matrix ornamented PCL scaffold for bone tissue engineering

作者全名："WANG, X. I. A. O. F. A. N. G.; TU, X. I. A. O. L. I. N.; MA, Y. U. F. E., I; CHEN, J. I. E.; SONG, Y. A. N. G.; LIU, G. U. A. N. G. L. I. A. N. G."

作者地址："[WANG, X. I. A. O. F. A. N. G.; TU, X. I. A. O. L. I. N.; MA, Y. U. F. E., I; CHEN, J. I. E.; LIU, G. U. A. N. G. L. I. A. N. G.] Chongqing Med Univ, Inst Life Sci, Lab Skeletal Dev & Regenerat, Chongqing 400016, Peoples R China; [TU, X. I. A. O. L. I. N.] Indiana Univ, Dept Anat & Cell Biol, Sch Med, Indianapolis, IN 46202 USA; [SONG, Y. A. N. G.] Chongqing Univ, Bioengn Coll, Chongqing 400044, Peoples R China"

通信作者："TU, XL (通讯作者)，Chongqing Med Univ, Inst Life Sci, Lab Skeletal Dev & Regenerat, Chongqing 400016, Peoples R China."

来源：BIOCELL

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000806696600010

JCR分区：Q3

影响因子：1.2

年份：2022

卷号：46

期号：9

开始页：2089

结束页：2099

文献类型：Article

关键词：Wnt3a; Decellularized matrix; Bone marrow stromal cells; Osteogenic differentiation; Bone tissue engineering

摘要："The limited bioactivity of scaffold materials is an important factor that restricts the development of bone tissue engineering. Wnt3a activates the classic Wnt/beta-catenin signaling pathway which effects bone growth and development by the accumulation of beta-catenin in the nucleus. In this study, we fabricated 3D printed PCL scaffold with Wnt3a-induced murine bone marrow-derived stromal cell line ST2 decellularized matrix (Wnt3a-ST2-dCM-PCL) and ST2 decellularized matrix (ST2-dCM-PCL) by freeze-thaw cycle and DNase decellularization treatment which efficiently decellularized >90% DNA while preserved most protein. Compared to ST2-dCM-PCL, Wnt3a-ST2-dCM-PCL significantly enhanced newly-seeded ST2 proliferation, osteogenic differentiation and upregulated osteogenic marker genes alkaline phosphatase (Alp), Runx2, type I collagen (Col 1) and osteocalcin (Ocn) mRNA expression. After 14 days of osteogenic induction, Wnt3a-ST2-dCM-PCL promoted ST2 mineralization. These results demonstrated that Wnt3a-induced ST2 decellularized matrix improve scaffold materials' osteoinductivity and osteoconductivity."

基金机构：National Natural Science Foundation of China [82002310]; Chongqing Postgraduate Research and Innovation Project [CYB20167]; Chongqing Postdoctoral Science Foundation [csts2019jcyj-bsh0068]

基金资助正文："This work was supported by the National Natural Science Foundation of China (Grant No. U1601220), the National Natural Science Foundation of China (Grant No. 82002310), the Chongqing Postgraduate Research and Innovation Project (Grant No. CYB20167), the Chongqing Postdoctoral Science Foundation (Grant No. csts2019jcyj-bsh0068)."