A Target-Feedback Rolling-Cleavage signal amplifier for ultrasensitive electrochemical detection of miRNA with Self-Assembled CeO2@Ag hybrid nanoflowers

作者全名："Liu, Qian; Shen, Huawei; Li, Baiying; Cai, Juan; Peng, Yang; Weng, Zhi; Yu, Hongyan; Xie, Guoming; Feng, Wenli"

作者地址："[Liu, Qian; Shen, Huawei; Li, Baiying; Weng, Zhi; Yu, Hongyan; Xie, Guoming; Feng, Wenli] Chongqing Med Univ, Dept Lab Med, Key Lab Med Diagnost Minist Educ, Chongqing 400016, Peoples R China; [Liu, Qian; Xie, Guoming] Chongqing Med Univ, SPRi Engn Res Ctr, Chongqing Med Lab Microfluid, Chongqing 400016, Peoples R China; [Cai, Juan] Army Med Univ, Southwest Hosp, Dept Clin Lab Med, Chongqing 400038, Peoples R China; [Peng, Yang] Chongqing Univ Canc Hosp, Clin Lab Med Ctr, Chongqing 400030, Peoples R China; [Feng, Wenli] Chongqing Med Univ, Dept Lab Med, Key Lab Med Diagnost Minist Educ, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China; [Xie, Guoming] Chongqing Med Univ, SPRi Engn Res Ctr, Dept Lab Med, Key Lab Med Diagnost Minist Educ, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China"

通信作者："Xie, GM; Feng, WL (通讯作者)，Chongqing Med Univ, Dept Lab Med, Key Lab Med Diagnost Minist Educ, Chongqing 400016, Peoples R China.; Xie, GM (通讯作者)，Chongqing Med Univ, SPRi Engn Res Ctr, Chongqing Med Lab Microfluid, Chongqing 400016, Peoples R China.; Feng, WL (通讯作者)，Chongqing Med Univ, Dept Lab Med, Key Lab Med Diagnost Minist Educ, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China.; Xie, GM (通讯作者)，Chongqing Med Univ, SPRi Engn Res Ctr, Dept Lab Med, Key Lab Med Diagnost Minist Educ, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China."

来源：BIOELECTROCHEMISTRY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000806801200005

JCR分区：Q1

影响因子：5

年份：2022

卷号：146

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Electrochemical biosensor; CeO2@Ag hybrid nanoflowers; Target-feedback rolling-cleavage signal ampli-fier; MicroRNAs

摘要："Currently, developing an effective and easy-to-operate signal amplification assay to detect the trace-amount miRNAs in serum remains a significant challenge. Herein, an ultrasensitive CeO2@Ag hybrid nanoflower (CeO2@Ag HNF)-labeled electrochemical biosensor was developed for sensing miRNA, based on a target -feedback rolling-cleavage (TFRC) signal amplifier. CeO2@Ag HNFs possessing a unique three-dimensional layered structure were synthesized without any complex reaction conditions, such as heating and vacuum. The bimetallic nanoflowers provided a large surface area and excellent CAT-like activity, thereby enhancing electrochemical performance. Based on the principle of branched catalytic hairpin assembly, target miRNA could continuously trigger the assembly of branched junctions with ends composed of DNAzyme. The activated DNAzyme was able to cleave the hairpin substrate efficiently and release to capture more CeO2@Ag HNFs label. The process combining target-driven branched catalytic hairpin assembly and DNAzyme-assisted rolling cleavage were defined as TFRC signal amplification. This sensitive electrochemical biosensor exhibited good linear relationship of 1 fM -1 nM with a detection limit down to 0.89 fM. The proposed method is expected to have a promising application in the miRNA-associated fundamental research and clinical diagnosis."

基金机构：National Natural Science Foundation of China [81902171]; Top Talent Project of Chongqing Medical University [BJRC202026]

基金资助正文：Acknowledgments This work was financially supported by the National Natural Science Foundation of China (No. 81902171) and Top Talent Project of Chongqing Medical University (BJRC202026) .