Surface plasma resonance biosensing of phosphorylated proteins via pH-adjusted specific binding of phosphate residues with UiO-66

作者全名："Luo, Nini; Shu, Yue; Zhu, Chuanlong; Cheng, Xiaoxue; Chen, Lixue; Wang, Jiang; Liu, Rui; Ju, Huangxian; Cheng, Wei; Xia, Qianfeng"

作者地址："[Luo, Nini; Zhu, Chuanlong; Liu, Rui; Xia, Qianfeng] Hainan Med Univ, Affiliated Hosp 2, Sch Trop Med, Key Lab Trop Translat Med,Minist Educ, Haikou 571199, Hainan, Peoples R China; [Luo, Nini; Shu, Yue; Cheng, Xiaoxue; Cheng, Wei] Chongqing Med Univ, Ctr Clin Mol Med Detect, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Chen, Lixue; Wang, Jiang] Chongqing Med Univ, Lab Res Ctr, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Ju, Huangxian] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Peoples R China"

通信作者："Xia, QF (通讯作者)，Hainan Med Univ, Affiliated Hosp 2, Sch Trop Med, Key Lab Trop Translat Med,Minist Educ, Haikou 571199, Hainan, Peoples R China.; Cheng, W (通讯作者)，Chongqing Med Univ, Ctr Clin Mol Med Detect, Affiliated Hosp 1, Chongqing 400016, Peoples R China."

来源：CHEMICAL ENGINEERING JOURNAL

ESI学科分类：ENGINEERING

WOS号：WOS:000807019600003

JCR分区：Q1

影响因子：15.1

年份：2022

卷号：446

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Surface plasma resonance; Metal-organic framework; UiO-66; Biosensor; Protein phosphorylation

摘要："The abnormal protein phosphorylation is closely related to a series of diseases including cancers, neurodegeneration, and other immune/inflammatory disorders. However, a simple, accurate, and pragmatic methodology for the direct detection of phosphorylation levels of target proteins is largely unavailable. Herein, a surface plasma resonance (SPR) biosensor was developed to detect phosphorylated protein based on significant SPR enhancement using UiO-66 and its high affinity for phosphate residues. Furthermore, the pH of the incubation buffer and the NaOH concentration in the washing solution to eliminate nonspecific adsorption of nanoparticles on the chip surface were optimized to adjust the surface charges of UiO-66. This improved the specificity and sensitivity of the proposed method. The designed biosensor could distinguish 1% phosphorylated peptides from non-phosphorylated peptides background. Moreover, the method could directly analyze the phosphorylation level of target protein in complex samples such as cell or tissue extracts without requiring a purification step in a single workflow. This simple and robust biosensor is a sensitive, accurate, and pragmatic toolbox to detect phosphorylated proteins without destroying the protein structure and can significantly facilitate phosphoproteomics and clinical diagnosis research."

基金机构："National Natural Science Foundation of China [81572080, 81873972]; Hainan Province Clinical Medical Center, Science and Technology Plan of Hainan Province [cstc2015shmszx120086]; Hainan Province Clinical Medical Center; Science and Technology Plan of Hainan Province; Natural Science Foundation Project of Chongqing [cstc2015shmszx120086]; Chongqing Science Fund for Distinguished Young Scholars [cstc2015shmszx120086]; Foundation for Innovative Research Groups of Chongqing Higher Education Institutions [cstc2019jcyjjqX0028]; [CXQT20013]"

基金资助正文："This work was funded by the National Natural Science Foundation of China (81572080 and 81873972) , Hainan Province Clinical Medical Center, Science and Technology Plan of Hainan Province (Clinical Research Center) , the Natural Science Foundation Project of Chongqing (cstc2015shmszx120086) , and the Chongqing Science Fund for Distinguished Young Scholars (cstc2019jcyjjqX0028) . The Foundation for Innovative Research Groups of Chongqing Higher Education Institutions (CXQT20013) . The authors would like to thank all the reviewers who participated in the review and MJEditor ( www.mjeditor.com ) for its linguistic assistance during the preparation of this article."