Integration of Bioglass Into PHBV-Constructed Tissue-Engineered Cartilages to Improve Chondrogenic Properties of Cartilage Progenitor Cells

作者全名："Xue, Ke; Zhang, Shuqi; Ge, Jin; Wang, Qiang; Qi, Lin; Liu, Kai"

作者地址："[Xue, Ke; Liu, Kai] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Plast & Reconstruct Surg, Sch Med, Shanghai, Peoples R China; [Xue, Ke] Hainan Western Cent Hosp, Dept Burn & Plast Surg, Hainan, Peoples R China; [Zhang, Shuqi] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Stomatol Hosp, Chongqing, Peoples R China; [Ge, Jin] Shanghai Jiao Tong Univ, Shanghai Key Lab Stomatol, Sch Med, Shanghai, Peoples R China; [Wang, Qiang] China Med Univ, Sch & Hosp Stomatol, Liaoning Prov Key Lab Oral Dis, Shenyang, Peoples R China; [Qi, Lin] Fudan Univ, Dept Radiol, Huadong Hosp, Shanghai, Peoples R China; [Ge, Jin] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Shanghai Res Inst Stomatol, Coll Stomatol,Sch Med,Dept Oral Surg, Shanghai, Peoples R China"

通信作者："Liu, K (通讯作者)，Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Plast & Reconstruct Surg, Sch Med, Shanghai, Peoples R China.; Qi, L (通讯作者)，Fudan Univ, Dept Radiol, Huadong Hosp, Shanghai, Peoples R China."

来源：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

ESI学科分类：Multidisciplinary

WOS号：WOS:000807461500001

JCR分区：Q1

影响因子：5.7

年份：2022

卷号：10

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：cartilage progenitor cells; PHBV; Bioglass; hydrophilicity; cartilage engineering

摘要："Background: The Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffold has proven to be a promising three-dimensional (3D) biodegradable and bioactive scaffold for the growth and proliferation of cartilage progenitor cells (CPCs). The addition of Bioglass into PHBV was reported to increase the bioactivity and mechanical properties of the bioactive materials.Methods: In the current study, the influence of the addition of Bioglass into PHBV 3D porous scaffolds on the characteristics of CPC-based tissue-engineered cartilages in vivo were compared. CPCs were seeded into 3D macroporous PHBV scaffolds and PHBV/10% Bioglass scaffolds. The CPC-scaffold constructs underwent 6 weeks in vitro chondrogenic induction culture and were then transplanted in vivo for another 6 weeks to evaluate the difference between the CPC-PHBV construct and CPC-PHBV/10% Bioglass construct in vivo.Results: Compared with the pure PHBV scaffold, the PHBV/10% Bioglass scaffold has better hydrophilicity and a higher percentage of adhered cells. The CPC-PHBV/10%Bioglass construct produced much more cartilage-like tissues with higher cartilage-relative gene expression and cartilage matrix protein production and better biomechanical performance than the CPC-PHBV construct.Conclusion: The addition of Bioglass into 3D PHBV macroporous scaffolds improves the characteristics of CPC-based tissue-engineered cartilages in vivo."

基金机构：Hainan Province natural Science Foundation of China [822CXTD537]; National Natural Science Foundation of China [81801929]

基金资助正文：Funding This work was supported by Hainan Province natural Science Foundation of China (822CXTD537) and the National Natural Science Foundation of China (81801929).