Biomechanical Effects of 3D-Printed Bioceramic Scaffolds With Porous Gradient Structures on the Regeneration of Alveolar Bone Defect: A Comprehensive Study

作者全名："Yang, Zhuohui; Wang, Chunjuan; Gao, Hui; Jia, Lurong; Zeng, Huan; Zheng, Liwen; Wang, Chao; Zhang, Hongmei; Wang, Lizhen; Song, Jinlin; Fan, Yubo"

作者地址："[Yang, Zhuohui; Wang, Chunjuan; Gao, Hui; Jia, Lurong; Zeng, Huan; Zheng, Liwen; Wang, Chao; Zhang, Hongmei; Song, Jinlin] Chongqing Med Univ, Stomatol Hosp, Chongqing, Peoples R China; [Yang, Zhuohui; Wang, Chunjuan; Jia, Lurong; Zhang, Hongmei; Song, Jinlin] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Gao, Hui; Zeng, Huan; Zheng, Liwen] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China; [Wang, Chao; Wang, Lizhen; Fan, Yubo] Beihang Univ, Beijing Adv Innovat Ctr Biomed Engn, Sch Biol Sci & Med Engn, Sch Engn Med,Key Lab Biomech & Mechanobiol,Minist, Beijing, Peoples R China"

通信作者："Wang, C; Zhang, HM (通讯作者)，Chongqing Med Univ, Stomatol Hosp, Chongqing, Peoples R China.; Zhang, HM (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China.; Wang, C (通讯作者)，Beihang Univ, Beijing Adv Innovat Ctr Biomed Engn, Sch Biol Sci & Med Engn, Sch Engn Med,Key Lab Biomech & Mechanobiol,Minist, Beijing, Peoples R China."

来源：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

ESI学科分类：Multidisciplinary

WOS号：WOS:000808266900001

JCR分区：Q1

影响因子：5.7

年份：2022

卷号：10

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：alveolar bone defect; bone scaffold; bioceramic; additive manufacture; biomimetics

摘要："In the repair of alveolar bone defect, the microstructure of bone graft scaffolds is pivotal for their biological and biomechanical properties. However, it is currently controversial whether gradient structures perform better in biology and biomechanics than homogeneous structures when considering microstructural design. In this research, bioactive ceramic scaffolds with different porous gradient structures were designed and fabricated by 3D printing technology. Compression test, finite element analysis (FEA) revealed statistically significant differences in the biomechanical properties of three types of scaffolds. The mechanical properties of scaffolds approached the natural cancellous bone, and scaffolds with pore size decreased from the center to the perimeter (GII) had superior mechanical properties among the three groups. While in the simulation of Computational Fluid Dynamics (CFD), scaffolds with pore size increased from the center to the perimeter (GI) possessed the best permeability and largest flow velocity. Scaffolds were cultured in vitro with rBMSC or implanted in vivo for 4 or 8 weeks. Porous ceramics showed excellent biocompatibility. Results of in vivo were analysed by using micro-CT, concentric rings and VG staining. The GI was superior to the other groups with respect to osteogenicity. The Un (uniformed pore size) was slightly inferior to the GII. The concentric rings analysis demonstrated that the new bone in the GI was distributed in the periphery of defect area, whereas the GII was distributed in the center region. This study offers basic strategies and concepts for future design and development of scaffolds for the clinical restoration of alveolar bone defect."

基金机构："National Natural Science Foundation of China [81870758, 12072055, 11872135, 12172034, U20A20390]; Natural Science Foundation of Beijing [L212063]; Natural Science Foundation of Chongqing [cstc2021jcyj-msxmX0560]; Chongqing Health Commission [2021MSXM209]; Bureau of Science and Technology of Yubei, Chongqing [2021(49)]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (Grant No. 81870758, 12072055, 11872135, 12172034, and U20A20390), Natural Science Foundation of Beijing (Grant No. L212063), Natural Science Foundation of Chongqing (#cstc2021jcyj-msxmX0560 to HZ), Chongqing Health Commission (No. 2021MSXM209 to HZ), Bureau of Science and Technology of Yubei, Chongqing (#2021(49) to HZ)."