Dual-Template Magnetic Molecularly Imprinted Polymer for Simultaneous Determination of Spot Urine Metanephrines and 3-Methoxytyramine for the Diagnosis of Pheochromocytomas and Paragangliomas

作者全名："Zeng, Hongyu; Zhang, Xiaoqing; Zhen, Qianna; He, Yifan; Wang, Haoran; Zhu, Yang; Sun, Qi; Ding, Min"

作者地址："[Zeng, Hongyu; Zhang, Xiaoqing; Wang, Haoran; Zhu, Yang; Sun, Qi; Ding, Min] Chongqing Med Univ, Sch Lab Med, Key Lab Lab Med Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Zhen, Qianna; He, Yifan] Chongqing Med Univ, Dept Endocrinol, Affiliated Hosp 1, Chongqing 400016, Peoples R China"

通信作者："Ding, M (通讯作者)，Chongqing Med Univ, Sch Lab Med, Key Lab Lab Med Diagnost, Minist Educ, Chongqing 400016, Peoples R China."

来源：MOLECULES

ESI学科分类：CHEMISTRY

WOS号：WOS:000808763500001

JCR分区：Q2

影响因子：4.6

年份：2022

卷号：27

期号：11

开始页：　

结束页：　

文献类型：Article

关键词：magnetic molecularly imprinted polymer; normetanephrine; metanephrine; 3-methoxytyramine; spot urine; PPGLs diagnosis

摘要："A novel dual-template magnetic molecularly imprinted polymer (MMIP) was synthesized to extract normetanephrine (NMN), metanephrine (MN) and 3-methoxytyramine (3-MT) from spot urine samples. As the adsorbent of dispersive solid-phase extraction (d-SPE), the MMIP was prepared using dopamine and MN as dual templates, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the crosslinking reagent and magnetic nanoparticles as the magnetic core. NMN, MN, 3-MT and creatinine (Cr) in spot urine samples were selectively enriched by d-SPE and detected by HPLC-fluorescence detection/ultraviolet detection. The peak area (A) ratios of NMN, MN and 3-MT to Cr were used for the diagnosis of pheochromocytomas and paragangliomas (PPGLs). The results showed that the adsorption efficiencies of MMIP for target analytes were all higher than 89.0%, and the coefficient variation precisions of intra-assay and inter-assay for the analytes were within 4.9% and 6.3%, respectively. The recoveries of the analytes were from 93.2% to 112.8%. The MMIP was still functional within 14 days and could be reused at least seven times. The d-SPE and recommended solid-phase extraction (SPE) were both used to pretreat spot urine samples from 18 PPGLs patients and 22 healthy controls. The correlation coefficients of A(NMN)/A(Cr) and A(MN)/A(Cr) between d-SPE and SPE were both higher than 0.95. In addition, the areas under the receiver operator curves for spot urine A(NMN)/A(Cr), A(MN)/A(Cr) and plasma free NMN and MN were 0.975, 0.773 and 0.990, 0.821, respectively, indicating the two methods had the similar performances. The d-SPE method took only 20 min, which was effective in clinical application."

基金机构："Natural Science Foundation of Chongqing, China [cstc2019jcyj-msxmX0103, cstc2020jcyj-msxmX0141]"

基金资助正文："This work was sponsored by the Natural Science Foundation of Chongqing, China (cstc2019jcyj-msxmX0103, cstc2020jcyj-msxmX0141)."