Calycosin prevents IL-1 beta-induced articular chondrocyte damage in osteoarthritis through regulating the PI3K/AKT/FoxO1 pathway

作者全名："Guo, Xiang; Pan, Xiaoyu; Wu, Jianhong; Li, Yuanzhou; Nie, Na"

作者地址："[Guo, Xiang; Wu, Jianhong] Shaoxing Univ, Sch Med, Shaoxing 312000, Zhejiang, Peoples R China; [Pan, Xiaoyu] Shaoxing Univ, Dept Clin Med, Med Coll, Shaoxing 312000, Zhejiang, Peoples R China; [Li, Yuanzhou] Shaoxing Geke Biol Technol Co Ltd, Shaoxing 312000, Zhejiang, Peoples R China; [Nie, Na] Chongqing Med Univ, Trauma Joint Surg, Affiliated Hosp 3, Chongqing 404100, Peoples R China"

通信作者："Guo, X (通讯作者)，Shaoxing Univ, Sch Med, Shaoxing 312000, Zhejiang, Peoples R China."

来源：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000811459900001

JCR分区：Q3

影响因子：2.1

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Osteoarthritis; Calycosin; Chondrocyte; PI3K; AKT; FoxO1; Apoptosis; Inflammation; ECM degradation

摘要："Osteoarthritis (OA) is a joint disorder that is associated with chondrocyte damage under inflammatory environment. Calycosin is an astragalus extract with potential anti-inflammatory and anti-tumor activities. The purpose of this research is to explore the activity and mechanism of calycosin in interleukin-1beta (IL-1 beta)-induced chondrocyte injury. In the present study, the targets of calycosin and OA were analyzed according to HERB, DisGeNet, String, GO terms, and KEGG pathway enrichment assays. Human primary chondrocytes were treated with calycosin, and stimulated with IL-1 beta. Cell viability was detected by CCK-8 assay. Cell apoptosis was investigated by flow cytometry, and caspase-3 activity analyses. Inflammation was analyzed according to inflammatory cytokines levels by enzyme-linked immunosorbent assay (ELISA). The proteins associated with extracellular matrix (ECM) degradation and phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/forkhead box O1 (FoxO1) signaling pathways were measured using Western blotting. The results showed that total of 25 overlapping targets of calycosin against OA were predicted. These targets might drive the FoxO pathway. Calycosin alone induced little cytotoxicity to chondrocytes, and it alleviated IL-1 beta-induced viability inhibition, cell apoptosis, inflammatory cytokine secretion, and ECM degradation in chondrocytes. Calycosin repressed IL-1 beta-induced activation of the PI3K/AKT/FoxO1 signaling. Activation of the PI3K/AKT/FoxO1 signaling mitigated the suppressive effect of calycosin on chondrocyte apoptosis, inflammation, and ECM degradation induced by IL-1 beta. As a conclusion, calycosin prevents IL-1 beta-induced chondrocyte apoptosis, inflammation, and ECM degradation through inactivating the PI3K/AKT/FoxO1 pathway."

基金机构：Research Initiation Project of Shaoxing University of Arts and Sciences [20210004]; National College Students Innovation and Entrepreneurship Training Program in 2020 [202010349051X]

基金资助正文：This research was supported by Research Initiation Project of Shaoxing University of Arts and Sciences (No. 20210004) and the National College Students Innovation and Entrepreneurship Training Program in 2020 (No. 202010349051X)