Dynamic Measurements of Cerebral Blood Flow Responses to Cortical Spreading Depolarization in the Murine Endovascular Perforation Subarachnoid Hemorrhage Model

作者全名："Yan, Jin; Li, Wenlang; Zhou, Chao; Wu, Na; Yang, Xiaomin; Pan, Qiuling; He, Tao; Wu, Yue; Guo, Zongduo; Xia, Yongzhi; Sun, Xiaochuan; Cheng, Chongjie"

作者地址："[Yan, Jin; Zhou, Chao; Wu, Na; Yang, Xiaomin; Pan, Qiuling; Wu, Yue; Guo, Zongduo; Xia, Yongzhi; Sun, Xiaochuan; Cheng, Chongjie] Chongqing Med Univ, Dept Neurosurg, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China; [Li, Wenlang] Chongqing Med Univ, Dept Anesthesiol, Affiliated Hosp 2, Chongqing, Peoples R China; [He, Tao] Chongqing Med Univ, Dept Orthopaed, Affiliated Hosp 1, Chongqing, Peoples R China"

通信作者："Sun, XC; Cheng, CJ (通讯作者)，Chongqing Med Univ, Dept Neurosurg, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：TRANSLATIONAL STROKE RESEARCH

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000815431900001

JCR分区：Q1

影响因子：6.9

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Subarachnoid hemorrhage; Delayed cerebral ischemia; Cerebral blood flow; Cortical spreading depolarization; Cortical spreading ischemia; Local field potential

摘要："Delayed cerebral ischemia (DCI) is the most severe complication after subarachnoid hemorrhage (SAH), and cortical spreading depolarization (CSD) is believed to play a vital role in it. However, the dynamic changes in cerebral blood flow (CBF) in response to CSD in typical SAH models have not been well investigated. Here, SAH was established in mice with endovascular perforation. Subsequently, the spontaneous CBF dropped instantly and then returned to baseline rapidly. After KCl application to the cortex, subsequent hypoperfusion waves occurred across the groups, while a lower average perfusion level was found in the SAH groups (days 1-7). Moreover, in the SAH groups, the number of CSD decreased within day 7, and the duration and spreading velocity of the CSD increased within day 3 and day 14, respectively. Next, we continuously monitored the local field potential (LFP) in the prefrontal cortex. The results showed that the decrease in the percentage of gamma oscillations lasted throughout the whole process in the SAH group. In the chronic phase after SAH, we found that the mice still had cognitive deficits but experienced no obvious tissue damage. In summary, SAH negatively affects the CBF responses to CSD and the spontaneous LFP activity and causes long-term cognitive deficits in mice. Based on these findings, in the specific phase after SAH, DCI is induced or exacerbated more easily by potential causers of CSD in clinical practice (edema, erythrocytolysis, inflammation), which may lead to neurological deterioration."

基金机构："National Natural Science Foundation of China [82071332, 82172193]; Natural Science Foundation of Chongqing, China [cstc2019ycyj-msxmX0830]"

基金资助正文："The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was sponsored by the National Natural Science Foundation of China, No. 82172193 to Dr Cheng; the National Natural Science Foundation of China, No. 82071332 to Dr Guo; and the Natural Science Foundation of Chongqing, China, No. cstc2019ycyj-msxmX0830 to Dr Xia."