Alterations of RNA Modification in Mouse Germ Cell-2 Spermatids Under Hypoxic Stress

作者全名："He, Tong; Guo, Huanping; Xia, Lin; Shen, Xipeng; Huang, Yun; Wu, Xiao; Jiang, Xuelin; Xu, Yinying; Tan, Yi; Zhang, Yunfang; Tan, Dongmei"

作者地址："[He, Tong; Shen, Xipeng; Huang, Yun; Tan, Dongmei] Chongqing Med Univ, Lab Anim Ctr, Chongqing, Peoples R China; [He, Tong; Guo, Huanping; Xia, Lin; Shen, Xipeng; Wu, Xiao; Jiang, Xuelin; Xu, Yinying; Zhang, Yunfang] Army Med Univ, Xinqiao Hosp, Med Ctr Hematol, Chongqing, Peoples R China; [Zhang, Yunfang] Tongji Univ, Clin & Translat Res Ctr Shanghai Matern & Infant H, Sch Life Sci & Technol, Shanghai Key Lab Signaling & Dis Res, Shanghai, Peoples R China"

通信作者："Tan, DM (通讯作者)，Chongqing Med Univ, Lab Anim Ctr, Chongqing, Peoples R China.; Zhang, YF (通讯作者)，Army Med Univ, Xinqiao Hosp, Med Ctr Hematol, Chongqing, Peoples R China.; Zhang, YF (通讯作者)，Tongji Univ, Clin & Translat Res Ctr Shanghai Matern & Infant H, Sch Life Sci & Technol, Shanghai Key Lab Signaling & Dis Res, Shanghai, Peoples R China."

来源：FRONTIERS IN MOLECULAR BIOSCIENCES

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000817143700001

JCR分区：Q2

影响因子：5

年份：2022

卷号：9

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：RNA modification; hypoxic stress; GC-2spd cells; spermatogenesis; high-throughput sequencing; mass spectrometry

摘要："Hypoxia is a known stress factor in mammals and has been shown to potentially impair male fertility, which manifests as spermatogenic dysfunction and decreased semen quality. Studies have shown that RNA modifications, the novel post-transcriptional regulators, are involved in spermatogenesis, and hypoxia-induced alterations in RNA modification in testes and sperm cells may be associated with impaired spermatogenesis in mice. However, the molecular mechanisms via which RNA modifications influence spermatogenesis under hypoxic stress conditions are unclear. In this study, we generated a mouse Germ Cell-2 spermatid (GC-2spd) hypoxia model by culturing cells in a 1% O-2 incubator for 48 h or treating them with CoCl2 for 24 h. The hypoxia treatment significantly inhibited proliferation and induced apoptosis in GC-2spd cells. The RNA modification signatures of total RNAs (2 types) and differentially sized RNA fragments (7 types of approximately 80 nt-sized tRNAs; 9 types of 17-50 nt-sized sncRNAs) were altered, and tRNA stability was partially affected. Moreover, the expression profiles of sncRNAs, such as microRNAs, tsRNAs, rsRNAs, and ysRNAs, were significantly regulated, and this might be related to the alterations in RNA modification and subsequent transcriptomic changes. We comprehensively analyzed alterations in RNA modification signatures in total RNAs, tRNAs (approximately 80 nt), and small RNAs (17-50 nt) as well as the expression profiles of sncRNAs and transcriptomes in hypoxia-treated GC-2spd cells; our data suggested that RNA modifications may be involved in cellular responses under hypoxic stress conditions and could provide a basis for a better understanding of the molecular mechanisms underlying male infertility."

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