Ca2+-based neural activity recording for rapidly screening behavioral correlates of the claustrum in freely behaving mice

作者全名："Qin, Jing; Huang, Wu-shuang; Du, Hao-ran; Zhang, Chun-qing; Xie, Peng; Qin, Han"

作者地址："[Qin, Jing; Huang, Wu-shuang; Xie, Peng] Chongqing Med Univ, Dept Neurol, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Qin, Jing; Huang, Wu-shuang; Xie, Peng] Chongqing Med Univ, NHC Key Lab Diag & Treatment Brain Funct Dis, Chongqing 400016, Peoples R China; [Zhang, Chun-qing] Army Med Univ, Xinqiao Hosp, Dept Neurosurg, 183 Xinqiao Main St, Chongqing 400037, Peoples R China; [Du, Hao-ran; Qin, Han] Chongqing Univ, Ctr Neurointelligence, Sch Med, Chongqing 400044, Peoples R China"

通信作者："Xie, P (通讯作者)，Chongqing Med Univ, Dept Neurol, Affiliated Hosp 1, Chongqing 400016, Peoples R China.; Xie, P (通讯作者)，Chongqing Med Univ, NHC Key Lab Diag & Treatment Brain Funct Dis, Chongqing 400016, Peoples R China.; Qin, H (通讯作者)，Chongqing Univ, Ctr Neurointelligence, Sch Med, Chongqing 400044, Peoples R China."

来源：BIOMEDICAL RESEARCH-TOKYO

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000817234700003

JCR分区：Q4

影响因子：1.2

年份：2022

卷号：43

期号：3

开始页：81

结束页：89

文献类型：Article

关键词：　

摘要："The claustrum has been hypothesized to participate in high-order brain functions, but experimental studies to demonstrate these functions are currently lacking. Neural activity recording of the claustrum in freely-behaving animals allows for correlating claustral activities with specific behaviors. However, previously utilized methods for studying the claustrum make it difficult to monitor neural activity patterns of freely-behaving animals in real time. Here we applied fiber photometry to monitor Ca2+ activity in the claustrum of freely-behaving mice. Using this method, we were able to achieve Ca2+ activity recording in both anesthetized and freely-behaving mice. We found that the dynamics of Ca2+ activity depended on anesthesia levels. As compared to the use of genetically encoded Ca2+ indicators that requires a few weeks of virus-dependent expression, we used a synthetic fluorescent Ca2+-sensitive dye, Oregon green 488 BAPTA-1, that allows for rapidly screening neural activity of interest within a few hours that relates to certain behaviors. In this way, we found the correlation between Ca2+ activity and specific behaviors, such as approaching an object. Our work offers an effective method for recording neural activity in the claustrum and thus for rapidly screening any behavioral relevance of the claustrum in freely-behaving mice."

基金机构：National Natural Science Foundation of China [82171463]

基金资助正文：The authors are grateful to Ms. Jia Lou for help in composing and layout editing of the figures. This work was supported by grants from the National Natural Science Foundation of China (82171463) to CZ.