Fabrication of avidin-stabilized gold nanoclusters with dual emissions and their application in biosensing

作者全名："Tang, Zhenrong; Chen, Fengjiao; Wang, Dan; Xiong, Dongmei; Yan, Shaoying; Liu, Shengchun; Tang, Hua"

作者地址："[Tang, Zhenrong; Liu, Shengchun] Chongqing Med Univ, Affiliated Hosp 1, Dept Endocrine & Breast Surg, Chongqing 400042, Peoples R China; [Chen, Fengjiao] Guangshan Cty Peoples Hosp, Xinyang 465450, Henan, Peoples R China; [Wang, Dan; Tang, Hua] Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis, Key Lab Mol Biol Infect Dis,Dept Infect Dis,Minis, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China; [Xiong, Dongmei] Chongqing Med & Pharmaceu Coll, Nursing Sch, Chongqing 401331, Peoples R China; [Yan, Shaoying] Nanchang Univ, Affiliated Hosp 1, Dept Clin Lab, Nanchang 330006, Jiangxi, Peoples R China"

通信作者："Liu, SC (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Endocrine & Breast Surg, Chongqing 400042, Peoples R China.; Tang, H (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis, Key Lab Mol Biol Infect Dis,Dept Infect Dis,Minis, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China."

来源：JOURNAL OF NANOBIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000817312700003

JCR分区：Q1

影响因子：10.2

年份：2022

卷号：20

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Avidin-stabilized gold nanoclusters (Av-Au NCs); Protein-stabilized gold nanoclusters (Prot-Au NCs); Dual-functional; Biosensor; Detect

摘要："Protein-stabilized gold nanoclusters (Prot-Au NCs) have been widely used in biosensing and cell imaging owing to their excellent optical properties and low biotoxicity. However, several Prot-Au NCs reported in the literature do not retain the biological role of the protein, which greatly limits their ability to directly detect biomarkers. This study demonstrated for the first time the successful synthesis of dual-function avidin-stabilized gold nanoclusters (Av-Au NCs) using a one-pot method. The resulting Av-Au NCs exhibited intense blue and red emissions under 374 nm excitation. Furthermore, the Av-Au NCs retained the native functionality of avidin to bind to biotin. When DNA strands modified with biotin at both ends (i.e., linker chains) were mixed with Av-Au NCs, large polymers were formed, indicating that Av-Au NCs could achieve fluorescence signal amplification by interacting with biotin.Taking advantage of the aforementioned properties, we constructed a novel enzyme-free fluorescent biosensor based on the Av-Au NCs-biotin system to detect DNA. The designed fluorescent biosensor could detect target DNA down to 0.043 nM, with a wide line range from 0.2 nM to 20 mu M. Thus, these dual-functional Av-Au NCs were shown to be an excellent fluorescent material for biosensing."

基金机构：Chongqing Medical Scientific Research Project (Joint project of Chongqing Health Commission and Science and Technology Bureau) [2022MSXM003]; Nursing School of Chongqing Medical and Pharmaceutical College [ygz2019303]; Province Natural Science Foundation of Jiangxi Province [20202BABL216040]; Science and Technology Plan Project of the Education Department of Jiangxi Province [GJJ190008]

基金资助正文："The authors thank Chongqing Medical Scientific Research Project (Joint project of Chongqing Health Commission and Science and Technology Bureau, 2022MSXM003), the Nursing School of Chongqing Medical and Pharmaceutical College (ygz2019303), the Province Natural Science Foundation of Jiangxi Province (20202BABL216040), and the Science and Technology Plan Project of the Education Department of Jiangxi Province (GJJ190008) for financial support."