A beta 1-40 Oligomers Trigger Neutrophil Extracellular Trap Formation through TLR4-and NADPH Oxidase-Dependent Pathways in Age-Related Macular Degeneration

作者全名："Chen, Jinquan; Zhao, Long; Ding, Xuanheng; Wen, Yan; Wang, Lingda; Shu, Qinxin; Xie, Wenxi; Liu, Yanyao; Peng, H."

作者地址："[Chen, Jinquan; Zhao, Long; Ding, Xuanheng; Wen, Yan; Wang, Lingda; Shu, Qinxin; Xie, Wenxi; Liu, Yanyao; Peng, H.] Chongqing Med Univ, Dept Ophthalmol, Affiliated Hosp 1, Chongqing, Peoples R China; [Chen, Jinquan; Zhao, Long; Ding, Xuanheng; Wen, Yan; Wang, Lingda; Shu, Qinxin; Xie, Wenxi; Liu, Yanyao; Peng, H.] Chongqing Eye Inst, Chongqing Key Lab Ophthalmol, Chongqing, Peoples R China"

通信作者："Peng, H (通讯作者)，Chongqing Med Univ, Dept Ophthalmol, Affiliated Hosp 1, Chongqing, Peoples R China.; Peng, H (通讯作者)，Chongqing Eye Inst, Chongqing Key Lab Ophthalmol, Chongqing, Peoples R China."

来源：OXIDATIVE MEDICINE AND CELLULAR LONGEVITY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000817634400003

JCR分区：Q2

影响因子：7.31

年份：2022

卷号：2022

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Neutrophils participate in the advancement of the human innate immune system and respond to perceived endogenous and exogenous threats. As a response mechanism, neutrophil extracellular traps (NETs) form near pathogens and surrounding tissues during an immune response. Drusen is an important marker of Age-Related Macular Degeneration (AMD) and plays an important role in the course of AMD. A beta 1-40 is the main component of drusen. However, the relationship between NETs and AMD or A beta 1-40 is unclear. Here, we found elevated levels of NETs in the serum of AMD patients and elevated levels in the serum of mouse models. We also observed the accumulation of neutrophils in the mouse retina. In addition, the production of NETs was inhibited by PAD4 inhibitors, which can alleviate chronic inflammation. Moreover, we confirmed that A beta 1-40 can induce NETs formation via the Toll-like receptor 4 (TLR4) and neutrophil NADPH oxidase (NOX) pathways. Our study confirmed that the formation of NETs is induced by A beta 1-40, and the results suggest that NETs may play a vital role in AMD pathogenesis."

基金机构：National Natural Science Foundation of China [81670881]

基金资助正文：AcknowledgmentsThis study was supported by funds from the National Natural Science Foundation of China (81670881).