Green synthesized gold nanoparticles from Pseudobulbus Cremastrae seu Pleiones show efficacy against hepatic carcinoma potentially through immunoregulation

作者全名："Zhu, Junmo; Liu, Zijing; Pu, Youwei; Xu, Jie; Zhang, Sitong; Bao, Yixi"

作者地址："[Zhu, Junmo; Pu, Youwei; Xu, Jie; Zhang, Sitong; Bao, Yixi] Chongqing Med Univ, Dept Clin Lab, Affiliated Hosp 2, Chongqing, Peoples R China; [Liu, Zijing] Chongqing Med Univ, Dept Gastroenterol, Affiliated Hosp 3, Chongqing, Peoples R China"

通信作者："Bao, YX (通讯作者)，Chongqing Med Univ, Dept Clin Lab, Affiliated Hosp 2, Chongqing, Peoples R China."

来源：DRUG DELIVERY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000817774300001

JCR分区：Q1

影响因子：6

年份：2022

卷号：29

期号：1

开始页：1983

结束页：1993

文献类型：Article

关键词：Pseudobulbus Cremastrae seu Pleiones; gold nanoparticles; green synthesis; immune function; liver cancer

摘要："Nanobiotechnology, the interface between biology and nanotechnology, has recently emerged in full bloom in the medical field due to its minimal side-effects and high efficiency. To broaden the application of nanobiotechnology, we composed gold nanoparticles from the extract of Pseudobulbus Cremastrae seu Pleiones (PCSP) using an efficient and green procedure. The biosynthesized Au nanoparticles containing PCSP (PCSP-AuNPs) were characterized by UV-vis spectroscopic, transmission electron microscopy (TEM), atomic force microscopy (AFM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), and Energy Dispersive X-ray (EDAX). After verifying the stability of PCSP-AuNPs, we detected its biosafety and immune-modulatory effects on RAW264.7 in vitro using NO assay, ELISA (TNF-alpha, IL-12p70, and IL-1 beta), and CCK-8 test. Furthermore, we examined the direct in vitro effects of PCSP-AuNPs on hepatocellular carcinomas (HCCs). Finally, we evaluated the immune regulation of PCSP-AuNPs using a mouse model with H22-tumor by testing the index of immune organs, splenic lymphocyte proliferation, cytokines levels (TNF-alpha and IL-10), and the CD4+/CD8+ cell ratio in the peripheral blood. Immunohistochemical analyses including H&E and PCNA staining were performed to investigate the anti-cancer efficacy and biocompatibility of PCSP-AuNPs. We found that PCSP-AuNPs not just possessed low toxicity, but also improved the immune-mediated antitumor response as compared to PCSP alone, suggesting its potential as a novel and efficient drug for liver cancer therapy."

基金机构：Natural Science Foundation of Chongqing Province of China [cstc2021jcyj-msxmX0094]; Postgraduate research and innovation projects of Chongqing Municipal Education Commission [CYS21223]; National Natural Science Foundation of China [81773940]

基金资助正文："This research was supported by the Natural Science Foundation of Chongqing Province of China (No. cstc2021jcyj-msxmX0094), Postgraduate research and innovation projects of Chongqing Municipal Education Commission (No. CYS21223), and the National Natural Science Foundation of China (No. 81773940)."