Long non-coding RNA lncHUPC1 induced by FOXA1 promotes tumor progression by inhibiting apoptosis via miR-133b/SDCCAG3 in prostate cancer

作者全名："Liu, Miao; Shen, Ai; Zheng, Yongbo; Chen, Xiong; Wang, Leilei; Li, Ting; Ouyang, Xin; Yu, Xian; Sun, Hao; Wu, Xiaohou"

作者地址："[Liu, Miao; Zheng, Yongbo; Chen, Xiong; Ouyang, Xin; Wu, Xiaohou] Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, Chongqing 400030, Peoples R China; [Liu, Miao; Yu, Xian; Sun, Hao] Chongqing Univ, Gastrointestinal Canc Ctr, Canc Hosp, Hanyu 181, Chongqing 400030, Peoples R China; [Shen, Ai] Chongqing Univ, Affiliated Canc Hosp, Hepatobiliary & Pancreat Tumor Ctr, Chongqing, Peoples R China; [Wang, Leilei; Li, Ting] Chongqing Med Univ, Key Lab Lab Med Diagnost, Minist Educ, Chongqing, Peoples R China"

通信作者："Wu, XH (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, Chongqing 400030, Peoples R China.; Sun, H (通讯作者)，Chongqing Univ, Gastrointestinal Canc Ctr, Canc Hosp, Hanyu 181, Chongqing 400030, Peoples R China."

来源：AMERICAN JOURNAL OF CANCER RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000820205900004

JCR分区：Q2

影响因子：5.3

年份：2022

卷号：12

期号：6

开始页：2465

结束页：+

文献类型：Article

关键词：Prostate cancer; lncRNAHUPC1; miR-133b; SDCCAG3; FOXA1

摘要："Long non-coding RNAs (lncRNAs) were confirmed to be involved in regulating various malignant behaviors of tumor cells in prostate cancer (PCa). Using The Cancer Genome Atlas (TCGA) prostate adenocarcinoma datasets, several endogenous competing RNA (ceRNA) networks of lncRNA/miRNA/mRNA associated with the progression-free survival (PFS) and Gleason score (GS) were identified using bioinformatics analysis. lncRNA AC004447.2 (lncHUPC1, ENSG00000269131)/miR-133b/serologically defined colon cancer antigen 3 (SDCCAG3) was a newly identified ceRNA network that affected cell growth and apoptosis in PCa. Using q-PCR, lncHUPC1 and SDCCAG3 were found to be up-regulated in PCa cells, while miR-133b was down-regulated. The same results were found in tissue samples from 70 PCa cases. It was confirmed that the knockdown of lncHUPC1 increased the expression of miR-133b and decreased that of SDCCAG3, which further increased apoptosis and inhibited cell growth, while the miR-133b inhibitor partially reversed these effects. After transfection with miR-133b mimic after lncHUPC1-knockdown, the expression of miR-133b increased while that of SDCCAG3 reduced, and the apoptosis of the cells was more obvious and the growth of the cells was slower. Therefore, lncHUPC1 was confirmed to regulate SDCCAG3 by binding to miR-133b. Additionally, we found that the transcription factor Forkhead BoxA1 (FOXA1) directly bound to the promoter of lncHUPC1 to activate it. In conclusion, the ceRNA network of lncHUPC1/miR-133b/SDCCAG3 affected the growth and apoptosis of PCa cells, and FOXA1 may be involved in the process as a transcription factor of lncHUPC1."

基金机构："Technical Innovation and Application Development Project of Chongqing Science and Technology Bureau [cstc2019jscx-msxmX0144, cstc2018jscx-msybx0157]; Natural Science Foundation of Chongqing municipality [cstc2018jxj1130018]; Fundamental Research Funds for the Central Universities [2021CDJYGRH-015]"

基金资助正文："This study was supported by grants from Technical Innovation and Application Development Project of Chongqing Science and Technology Bureau (cstc2019jscx-msxmX0144 and cstc2018jscx-msybx0157), Natural Science Foundation of Chongqing municipality (cstc2018jxj1130018) and the Fundamental Research Funds for the Central Universities (No.2021CDJYGRH-015)."