A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes

作者全名："Chen, Wenqin; Zhang, Yan; Di, Kaili; Liu, Chang; Xia, Yanyan; Ding, Shijia; Shen, Han; Li, Zhiyang"

作者地址："[Chen, Wenqin; Zhang, Yan; Di, Kaili; Liu, Chang; Xia, Yanyan; Shen, Han; Li, Zhiyang] Nanjing Univ, Affiliated Hosp, Nanjing Drum Tower Hosp, Dept Clin Lab,Med Sch, Nanjing, Peoples R China; [Ding, Shijia] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing, Peoples R China"

通信作者："Shen, H; Li, ZY (通讯作者)，Nanjing Univ, Affiliated Hosp, Nanjing Drum Tower Hosp, Dept Clin Lab,Med Sch, Nanjing, Peoples R China.; Ding, SJ (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing, Peoples R China."

来源：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

ESI学科分类：Multidisciplinary

WOS号：WOS:000823712700001

JCR分区：Q1

影响因子：5.7

年份：2022

卷号：10

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：exosomes; fluorescence biosensing; aptamer; G4-hemin; tumor diagnosis

摘要："Traditional detection methods for protein tumor markers in the early screening of breast cancer are restricted by complicated operation procedures and unstable reproducibility. As one of alternative emerging tumor markers, exosomes play an important role in diagnosing and treating cancers at the early stage due to traceability of their origins and great involvement in occurrence and development of cancers. Herein, a washing-free and efficient fluorescent biosensor has been proposed to realize simple and straightforward analysis of breast cancer cell-derived exosomes based on high affinity aptamers and G quadruplex-hemin (G4-hemin). The whole reaction process can be completed by several simple steps, which realizes washing-free and labor-saving. With simplified operation procedures and high repeatability, the linear detection range for this developed fluorescent biosensing strategy to breast cancer cell-derived exosomes is from 2.5 x 10(5) to 1.00 x 10(7) particles/ml, and the limit of detection is down to 0.54 x 10(5) particles/ml."

基金机构："National Natural Science Foundation of China [61971216, 82002205]; Key Research and Development Project of Jiangsu Province [BE2019603, BE2020768, BE2019761]"

基金资助正文："Funding This study was supported by the National Natural Science Foundation of China (Nos. 61971216 and 82002205), the Key Research and Development Project of Jiangsu Province (Nos. BE2019603, BE2020768, and BE2019761)."