Comprehensive Metabolomic Profiling of Cord Blood and Placental Tissue in Surviving Monochorionic Twins Complicated by Twin-Twin Transfusion Syndrome With or Without Fetoscopic Laser Coagulation Surgery: A Retrospective Cohort Study

作者全名："Liu, Tianjiao; Wen, Li; Huang, Shuai; Han, Ting-Li; Zhang, Lan; Fu, Huijia; Li, Junnan; Tong, Chao; Qi, Hongbo; Saffery, Richard; Baker, Philip N.; Kilby, Mark D."

作者地址："[Liu, Tianjiao; Wen, Li; Huang, Shuai; Zhang, Lan; Li, Junnan; Tong, Chao; Qi, Hongbo] Chongqing Med Univ, Affiliated Hosp 1, State Key Lab Maternal & Fetal Med Chongqing Muni, Chongqing, Peoples R China; [Liu, Tianjiao; Wen, Li; Huang, Shuai; Han, Ting-Li; Zhang, Lan; Li, Junnan; Tong, Chao] Chongqing Med Univ, Int Collaborat Lab Reprod & Dev, Minist Educ, Chongqing, Peoples R China; [Wen, Li; Huang, Shuai; Zhang, Lan; Li, Junnan] Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet, Chongqing, Peoples R China; [Fu, Huijia] Chongqing Med Univ, Affiliated Hosp 1, Dept Reprod Hlth & Infertil, Chongqing, Peoples R China; [Qi, Hongbo] Chongqing Women & Childrens Hlth Ctr, Chongqing, Peoples R China; [Saffery, Richard] Murdoch Childrens Res Inst, Canc Dis & Dev Epigenet, Parkville, Vic, Australia; [Saffery, Richard] Univ Melbourne, Dept Pediat, Parkville, Vic, Australia; [Baker, Philip N.] Univ Leicester, Coll Life Sci, Leicester, Leics, England; [Kilby, Mark D.] Univ Birmingham, Inst Metab & Syst Res, Birmingham, W Midlands, England; [Kilby, Mark D.] Birmingham Womens & Childrens Fdn Trust, Fetal Med Ctr, Birmingham, W Midlands, England"

通信作者："Tong, C; Qi, HB (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, State Key Lab Maternal & Fetal Med Chongqing Muni, Chongqing, Peoples R China.; Tong, C (通讯作者)，Chongqing Med Univ, Int Collaborat Lab Reprod & Dev, Minist Educ, Chongqing, Peoples R China.; Qi, HB (通讯作者)，Chongqing Women & Childrens Hlth Ctr, Chongqing, Peoples R China."

来源：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

ESI学科分类：Multidisciplinary

WOS号：WOS:000825551600001

JCR分区：Q1

影响因子：5.7

年份：2022

卷号：10

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：twin-twin transfusion syndrome; fetoscopic laser coagulation; metabolomics; cord plasma; placenta

摘要："Objectives: To investigate metabolomic perturbations caused by twin-twin transfusion syndrome, metabolic changes associated with fetoscopic laser coagulation in both placental tissue and cord plasma, and to investigate differential metabolites pertinent to varying fetal outcomes, including hemodynamic status, birth weight, and cardiac function, of live-born babies. Methods: Placental tissue and cord plasma samples from normal term or uncomplicated preterm-born monochorionic twins and those complicated by twin-twin transfusion syndrome treated with or without fetoscopic laser coagulation were analyzed by high-performance liquid chromatography metabolomic profiling. Sixteen comparisons of different co-twin groups were performed. Partial least squares-discriminant analysis, metabolic pathway analysis, biomarker analysis, and Spearman's correlation analysis were conducted based on differential metabolites used to determine potential biomarkers in different comparisons and metabolites that are pertinent to neonatal birth weight and left ventricular ejection fraction. Results: These metabolomic investigations showed that the cord plasma metabolome has a better performance in discriminating fetuses among different hemodynamic groups than placental tissue. The metabolic alteration of twin-twin transfusion syndrome in these two types of samples centers on fatty acid and lipid metabolism. The fetoscopic laser coagulation procedure improves the metabolomic change brought by this syndrome, making the metabolomes of the treated group less distinguishable from those of the control and preterm birth groups. Certain compounds, especially lipids and lipid-like molecules, are noted to be potential biomarkers of this morbid disease and pertinent to neonatal birth weight and ejection fraction. Conclusions: Fetoscopic laser coagulation can ameliorate the metabolomic alteration caused by twin-twin transfusion syndrome in placental tissue and cord plasma, which are involved mainly in fatty acid and lipid-like molecule metabolism. Certain lipids and lipid-like molecules are helpful in differentiating co-twins of different hemodynamic statuses and are significantly correlated with neonatal birth weight or ejection fraction."

基金机构："National Natural Science Foundation of China [U21A20346, 82171662, 82001580]; Chongqing Science and Technology Commission [cstc2019jcyj-msxmX0856, cstc2021ycjh-bgzxm0192]; Chongqing Health Committee [2019GDRC012, 2020MSXM037]"

基金资助正文："This study was funded by the National Natural Science Foundation of China (U21A20346, 82171662 and 82001580), the Chongqing Science and Technology Commission (cstc2019jcyj-msxmX0856, cstc2021ycjh-bgzxm0192), and the Chongqing Health Committee (2019GDRC012, 2020MSXM037)."