EspF of Enterohemorrhagic Escherichia coli Enhances Apoptosis via Endoplasmic Reticulum Stress in Intestinal Epithelial Cells: An Isobaric Tags for Relative and Absolute Quantitation-Based Comparative Proteomic Analysis

作者全名："Wang, Xiangyu; Yan, Kaina; Fu, Muqing; Liang, Song; Zhao, Haiyi; Fu, Changzhu; Yang, Lan; Song, Zhihong; Sun, Dayong; Wan, Chengsong"

作者地址："[Wang, Xiangyu; Yang, Lan; Song, Zhihong; Sun, Dayong] Shenzhen Univ, Shenzhen Peoples Hosp 2, Dept Gastroenterol, Affiliated Hosp 1, Shenzhen, Peoples R China; [Yan, Kaina; Fu, Muqing; Liang, Song; Wan, Chengsong] Southern Med Univ, Sch Publ Hlth, BSL 3 Lab Guangdong, Guangdong Prov Key Lab Trop Dis Res,, Guangzhou, Peoples R China; [Yan, Kaina] Chongqing Med Univ, Inst Life Sci, Ctr Novel Target & Therapeut Intervent, Chongqing, Peoples R China; [Zhao, Haiyi] Genecreate Biol Engn Co Ltd, Natl Bioind Base, Wuhan, Peoples R China; [Fu, Changzhu] Univ Cambridge, Sch Biol Sci, MRC Toxicol Unit, Cambridge, England"

通信作者："Sun, DY (通讯作者)，Shenzhen Univ, Shenzhen Peoples Hosp 2, Dept Gastroenterol, Affiliated Hosp 1, Shenzhen, Peoples R China.; Wan, CS (通讯作者)，Southern Med Univ, Sch Publ Hlth, BSL 3 Lab Guangdong, Guangdong Prov Key Lab Trop Dis Res,, Guangzhou, Peoples R China."

来源：FRONTIERS IN MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000826210200001

JCR分区：Q2

影响因子：5.2

年份：2022

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Enterohemorrhagic Escherichia coli; EspF; endoplasmic reticulum stress; apoptosis; iTRAQ

摘要："There have been large foodborne outbreaks related to Enterohemorrhagic Escherichia coli (EHEC) around the world. Among its virulence proteins, the EspF encoded by locus of enterocyte effacement is one of the most known functional effector proteins. In this research, we infected the HT-29 cells with the EHEC wild type strain and EspF-deficient EHEC strain. Via the emerging technique isobaric tags for relative and absolute quantitation (iTRAQ), we explored the pathogenic characteristics of EspF within host cells. Our data showed that the differences regarding cellular responses mainly contained immune regulation, protein synthesis, signal transduction, cellular assembly and organization, endoplasmic reticulum (ER) stress, and apoptosis. Notably, compared with the EspF-deficient strain, the protein processing in the ER and ribosome were upregulated during wild type (WT) infection. Our findings proved that the EspF of Enterohemorrhagic Escherichia coli induced ER stress in intestinal epithelial cells; the ER stress-dependent apoptosis pathway was also activated within the host cells. This study provides insight into the virulence mechanism of protein EspF, which will deepen our general understanding of A/E pathogens and their interaction with host proteins."

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