Highly sensitive electrochemical aptasensor for SARS-CoV-2 antigen detection based on aptamer-binding induced multiple hairpin assembly signal amplification

作者全名："Xue, Jian; Li, Ying; Liu, Jie; Zhang, Zixuan; Yu, Rongjun; Huang, Yaling; Li, Chaorui; Chen, Anyi; Qiu, Jingfu"

作者地址："[Xue, Jian; Li, Ying; Liu, Jie; Zhang, Zixuan; Yu, Rongjun; Huang, Yaling; Li, Chaorui; Chen, Anyi; Qiu, Jingfu] Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 400016, Peoples R China; [Xue, Jian] Zunyi Med & Pharmaceut Coll, Hlth Management Dept, Zunyi 563006, Guizhou, Peoples R China"

通信作者："Chen, AY; Qiu, JF (通讯作者)，Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 400016, Peoples R China."

来源：TALANTA

ESI学科分类：CHEMISTRY

WOS号：WOS:000826716700004

JCR分区：Q1

影响因子：6.1

年份：2022

卷号：248

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Aptamer/protein proximity binding; Multiple hairpin assembly; SARS-CoV-2; Electrochemical aptasensor; Spike protein

摘要："In this work, a brief electrochemical aptasensor was developed for highly sensitive detection of SARS-CoV-2 antigen utilizing an aptamer-binding induced multiple hairpin assembly strategy for signal amplification. In the presence of SARS-CoV-2, a pair of aptamers was brought in a close proximity according to the aptamerprotein antigen binding, which initiated strand displacement reaction thereby triggering a multiple hairpin assembly to obtain long linear DNA concatemers on the electrode surface. As the fabricated hairpin probes were labeled with biotin, massive streptavidin-alkaline phosphatases (ST-ALP) could be further introduced on the electrode interface via biotin-streptavidin interaction thus generating strong electrochemical signal in electrolyte solution containing 1-naphthol phosphate. Benefiting from the non-enzymatic multiple hairpin assembly signal amplification strategy, the designed aptasensor for SARS-CoV-2 spike protein detection exhibited the wide linear range from 50 fg.mL(-1 )to 50 ng.mL(-1 ) and low detection limit of 9.79 fg.mL(-1 ). Meaningfully, this proposed electrochemical assay provided a potential application for the point of care analysis of viral diseases under ambient temperature."

基金机构：Natural Science Foundation of Chongqing [cstc2020jcyj-zdxm0015]; China Postdoctoral Science Foundation [2020M683259]; Science and Technology Support Program of Guizhou Science and Technology Department [(2020)4Y183]; Science and Technology Support Program of Zunyi Science and Technology Bureau [NS(2020)22]

基金资助正文："This work was sponsored by Natural Science Foundation of Chongqing (cstc2020jcyj-zdxm0015) , China Postdoctoral Science Foundation (2020M683259) , the Science and Technology Support Program of Guizhou Science and Technology Department ( (2020)4Y183) and the Science and Technology Support Program of Zunyi Science and Technology Bureau [NS(2020)22] ."