Near-infrared probe for early diagnosis of diabetic complications-nephropathy and in vivo visualization fluorescence imaging research

作者全名："Wang, Jiemin; Zhang, Liang; Qin, Wenwu; Liu, Yun"

作者地址："[Wang, Jiemin; Qin, Wenwu] Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat G, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China; [Wang, Jiemin; Qin, Wenwu] Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Special Funct Mat & Struct Design MOE, Lanzhou 730000, Peoples R China; [Liu, Yun] Chongqing Med Univ, Dept Radiol, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Zhang, Liang] Army Med Univ, Xinqiao Hosp, Dept Radiol, Chongqing 400037, Peoples R China"

通信作者："Qin, WW (通讯作者)，Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat G, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China.; Qin, WW (通讯作者)，Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Special Funct Mat & Struct Design MOE, Lanzhou 730000, Peoples R China.; Liu, Y (通讯作者)，Chongqing Med Univ, Dept Radiol, Affiliated Hosp 2, Chongqing 400010, Peoples R China."

来源：ANALYTICA CHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:000830243400004

JCR分区：Q1

影响因子：6.2

年份：2022

卷号：1221

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Dipeptidylpeptidase4; Diabeticnephropathy; Diabeticmice; Invivoimaging; Near-infraredfluorescentprobe

摘要："Diabetic nephropathy is one of the common complications of diabetes, which has high risk of renal function. Dipeptidyl peptidase 4 (DPP4) is considered to be one of the good dynamic monitoring indicators for early diabetic nephropathy. Therefore, real-time monitoring of changes in the activity of DPP4 in organisms is helpful to the diagnosis and treatment of diabetes and its complications-diabetic nephropathy. A near-infrared fluo-rescent probe GP-DCMNH2 is designed to detect the activity of DPP4. GP-DCMNH2 is catalyzed and hydrolyzed by DPP4 into the near-infrared fluorescent dye DCMNH2, to achieve the purpose of detecting DPP4 in organisms. Based on the excellent near-infrared spectroscopy characteristics displayed by the probe GP-DCMNH2 in vitro, in living cells and diabetic mouse models, GP-DCMNH2 has been further applied in the visual fluorescence imaging of diabetic complications-diabetic nephropathy. Compared with the control group and the treatment group, GP-DCMNH2 showed a stronger near-infrared fluorescence signal in the kidney tissue and blood of diabetic ne-phropathy mice. Because GP-DCMNH2 shows high sensitivity in real-time dynamic monitoring of changes in the activity of DPP4 in organisms, and shows strong practicability in the spectral test of mouse models of diabetes and diabetic nephropathy. In clinical medicine, GP-DCMNH2 is expected to be used in the early diagnosis, prevention and treatment of diabetes and diabetic nephropathy."

基金机构："National Natural Science Foundation of China [21771092, 81903561]; Fundamental Research Funds for Central Universities [lzujbky-2021-kb17]; Special Fund Project of Guiding Scientific, technological Innovation Development of Gansu Province [2019ZX-04]; 111 Project [B20027]; State Key Laboratory of Veterinary Etiological Biology; Lanzhou Veterinary Research Institute; Chinese Academy of Agricultural Sciences, China"

基金资助正文："The authors would like to thank the National Natural Science Foundation of China (no. 21771092 and 81903561). This work was supported by the Fundamental Research Funds for the Central Universities (lzujbky-2021-kb17), the Special Fund Project of Guiding Scientific, technological Innovation Development of Gansu Province (2019ZX-04) and the 111 Project, B20027. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China, funded this work."