Aptasensor for Mycobacterium tuberculosis antigen MPT64 detection using anthraquinone derivative confined in ordered mesoporous carbon as a new redox nanoprobe

作者全名："Li, Yishi; Peng, Dengyong; Guo, Shuliang; Yang, Bijun; Zhou, Jing; Zhou, Jiaxu; Zhang, Qifan; Bai, Lijuan"

作者地址："[Li, Yishi; Peng, Dengyong; Guo, Shuliang; Yang, Bijun; Zhou, Jiaxu; Zhang, Qifan] Chongqing Med Univ, Affiliated Hosp 1, Dept Resp & Crit Care Med, Chongqing 400016, Peoples R China; [Peng, Dengyong; Zhou, Jing; Bai, Lijuan] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China"

通信作者："Bai, LJ (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China."

来源：BIOELECTROCHEMISTRY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000830913600003

JCR分区：Q1

影响因子：5

年份：2022

卷号：147

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Anthraquinone-2-carboxylic acid; Ordered mesoporous carbon; Tuberculosis; MPT64 antigen; Aptasensor

摘要："Rapid and sensitive tuberculosis (TB) diagnoses remain big challenges to current detection tools. In this work, a sensitive electrochemical aptasensor was constructed for the determination of Mycobacterium tuberculosis antigen MPT64 using a new redox nanoprobe. We found that anthraquinone derivative, anthraquinone-2-carboxylic acid (AQCA), a redox mediator, could be confined in ordered mesoporous carbon material of CMK-3. Due to the large loading amount of AQCA, as well as the confined space and electron transfer promotion effect of CMK-3, the obtained AQCA/CMK-3 nanohybrid with mass ratio of 2:1 showed excellent electroactivity and was employed as a new redox nanoprobe for signal amplification for the first time. Additionally, urchin-like Ce-MOFs were used to load a large amount of deposited gold nanocrystals (dep-Au), leading to dense immobilization of capture probe. The proposed electrochemical aptasensor for MPT64 detection showed a good linear relationship in the range from 100 fg/mL to 10 ng/mL with a low detection limit of 67.6 fg/mL. Besides, the aptasensor was utilized to detect MTP64 in human serum samples for TB diagnosis and presented satisfactory results."

基金机构："National Natural Science Foundation of China [82072378, 81601856]; Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]; Program for Youth Inno-vation in Future Medicine, Chongqing Medical University, China [YS2019020]; Ba Yu Scholar Program, China [W0067]; Program for Youth Innovation in Future Medicine, Chongqing Medical University, China [YS2019020]; Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China; Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [W0067]; Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City [YXY2019XSGG4]; [cstc2014kjrc-qnrc00004]"

基金资助正文："This work is supported by National Natural Science Foundation of China (82072378, 81601856) , Chongqing Talents: Exceptional Young Talents Project, China (CQYC202005015, cstc2021ycjh-bgzxm0328) , Ba Yu Scholar Program, China (YS2019020) , Program for Youth Innovation in Future Medicine, Chongqing Medical University, China (W0067) , Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019) , Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China (YXY2019XSGG4) and Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City (cstc2014kjrc-qnrc00004) ."