Reduced neutralization of SARS-CoV-2 B.1.617 variant by convalescent and vaccinated sera

作者全名："Hu, Jie; Wei, Xiao-yu; Xiang, Jin; Peng, Pai; Xu, Feng-li; Wu, Kang; Luo, Fei-yang; Jin, Ai -shun; Fang, Liang; Liu, Bei-zhong; Wang, Kai; Tang, Ni; Huang, Ai-Long"

作者地址："[Hu, Jie; Xiang, Jin; Peng, Pai; Xu, Feng-li; Wu, Kang; Wang, Kai; Tang, Ni; Huang, Ai-Long] Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis, Dept Infect Dis,Key Lab Mol Biol Infect Dis,Minist, Chongqing 400010, Peoples R China; [Wei, Xiao-yu; Fang, Liang; Liu, Bei-zhong] Chongqing Med Univ, Yong Chuan Hosp, Chongqing 402177, Peoples R China; [Luo, Fei-yang; Jin, Ai -shun] Chongqing Med Univ, Coll Basic Med, Dept Immunol, Chongqing 400016, Peoples R China"

通信作者："Wang, K; Tang, N; Huang, AL (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis, Dept Infect Dis,Key Lab Mol Biol Infect Dis,Minist, Chongqing 400010, Peoples R China.; Liu, BZ (通讯作者)，Chongqing Med Univ, Yong Chuan Hosp, Chongqing 402177, Peoples R China."

来源：GENES & DISEASES

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000831198700018

JCR分区：Q1

影响因子：6.8

年份：2022

卷号：9

期号：5

开始页：1290

结束页：1300

文献类型：Article

关键词：Coronavirus; Immune escape; Mutation; Neutralizing antibodies; SARS-CoV-2; Vaccine; Viral entry

摘要："Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syn-drome coronavirus 2 (SARS-CoV-2). The Spike protein that mediates coronavirus entry into host cells is a major target for COVID-19 vaccines and antibody therapeutics. However, multiple variants of SARS-CoV-2 have emerged, which may potentially compromise vaccine effective-ness. Using a pseudovirus-based assay, we evaluated SARS-CoV-2 cell entry mediated by the viral Spike B.1.617 and B.1.1.7 variants. We also compared the neutralization ability of mono-clonal antibodies from convalescent sera and neutralizing antibodies (NAbs) elicited by Coro-naVac (inactivated vaccine) and ZF2001 (RBD-subunit vaccine) against B.1.617 and B.1.1.7 variants. Our results showed that, compared to D614G and B.1.1.7 variants, B.1.617 shows enhanced viral entry and membrane fusion, as well as more resistant to antibody neutraliza-tion. These findings have important implications for understanding viral infectivity and for im-munization policy against SARS-CoV-2 variants. Copyright (c) 2021, Chongqing Medical University. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/ by-nc-nd/4.0/)."

基金机构："National Natural Science Foundation of China [U20A20392]; 111 Project [D20028]; Open Research Fund Program of the Key Laboratory of Molecular Biology for Infectious Diseases, China [CQMU202102, CQMU202105]; Science and Technology Research Program of Chongqing Municipal Education Commission, China [KJZD-M202000401]; Natural Science Foundation Project of Chongqing, China [cstc2019jscx-dxwtBX0019]; Emergency Project from the Science & Technology Commission of Chongqing, China [cstc2020jscx-fyzx0053, cstc2020jscx-dxwtB0050]; Kuanren Talents Program of the second affiliated hospital of Chongqing Medical University; Emergency Project for Novel Coronavirus Pneumonia from the Chongqing Medical University, China [CQMUNCP0302]; China Postdoctoral Science Foundation, China [2021M693924]; Chongqing Postdoctoral Science Special Foundation, China [2010010005216630]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (No. U20A20392), the 111 Project (No. D20028); Open Research Fund Program of the Key Laboratory of Molecular Biology for Infectious Diseases, China (No. CQMU202102 and CQMU202105); The Science and Technology Research Program of Chongqing Municipal Education Commission, China (No. KJZD-M202000401); The Natural Science Foundation Project of Chongqing, China (No. cstc2019jscx-dxwtBX0019); The Emergency Project from the Science & Technology Commission of Chongqing, China (No. cstc2020jscx-fyzx0053 and cstc2020jscx-dxwtB0050); Kuanren Talents Program of the second affiliated hospital of Chongqing Medical University, the Emergency Project for Novel Coronavirus Pneumonia from the Chongqing Medical University, China (No. CQMUNCP0302); China Postdoctoral Science Foundation, China (No. 2021M693924); and Chongqing Postdoctoral Science Special Foundation, China (No. 2010010005216630)."