Collagen scaffold loaded allogeneic neural stem cells promoted locomotion recovery of spinal cord injury mainly through secreting neurotrophic factors
作者全名："Liu, Shuo; Fan, Caixia; Xie, Yuanyuan; Wang, Liudi; Cui, Yanyan; Wang, Bin"
作者地址："[Liu, Shuo; Xie, Yuanyuan; Wang, Liudi; Wang, Bin] Nanjing Univ, Med Sch, Clin Stem Cell Ctr, Affiliated Drum Tower Hosp, Nanjing 210000, Peoples R China; [Fan, Caixia] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Div Nanobiomed, Key Lab Nanobio Interface Res, 398 Ruoshui Rd, Suzhou 215123, Peoples R China; [Cui, Yanyan] Chongqing Med Univ, Dept Cell Biol & Genet, Chongqing 400016, Peoples R China"
通信作者："Wang, B (通讯作者)，Zhongshan Rd, Nanjing 210008, Peoples R China.; Cui, YY (通讯作者)，1 Yixueyuan Rd, Chongqing 400016, Peoples R China."
来源：MATERIALS & DESIGN
关键词：Spinal cord injury; Neural stem cells; Neurotrophic factors; Collagen scaffold; Paracrine; Locomotion recovery
摘要："The combination of biological scaffold and neural stem cells (NSCs) is a promising treatment strategy for spinal cord injury (SCI). But adverse niche at injures spinal cord severe hamper the transplanted NSCs to preferentially differentiate neurons. In this study, we compared the therapeutic outcomes in rat completely transected SCI model after transplantations of collagen scaffold loaded with NSCs in different forms including unicellular NSCs, neurospheres, differentiated unicellular NSCs, and differentiated neurospheres. The results showed collagen scaffold combined each form of NSCs could markedly restore the motor function of the hindlimbs by Basso-Beattie-Bresnahan(BBB) scoring to similar levels. The transcriptome analysis showed there were similar expressions of neurotrophic factors among neurospheres, differentiated unicellular NSCs, and differentiated neurospere. Next, we enriched neurotrophic factors secreted from culture medium of these three forms of NSCs respectively and combined with collagen scaffold were transplanted into SCI to further assay therapeutic effects of secreting neurotrophic factors on SCI. BBB score analysis showed that the secreted neurotrophic factors from above three forms of NSCs had similar functional recovery of SCI. Therefore, we proposed that allogeneic NSCs transplant promotes functional recovery of SCI predominantly by secreting neurotrophic factors, not by direct neuronal replacement of differentiated neurons from transplanted cells. (C) 2022 The Authors. Published by Elsevier Ltd."
基金机构："National Key Research and Development Program of China [2017YFA0104304]; National Natural Science Foundation of China (NSFC) [81571213, 82070459, 81800583, 81901266]; Nanjing Medical Science and technique Development Foundation [QRX17006]; Nanjing Medical Science and Innovation Platform [ZDX16005]; Science and Technology Bureau of Yuzhong District ; Jiangsu Biobank of Clinical Resources [BM2015004]"
基金资助正文："This work was supported by the National Key Research and Development Program of China (2017YFA0104304), the National Natural Science Foundation of China (NSFC) (81571213, 82070459, Bin Wang, 81800583, Yuanyuan Xie, and 81901266, Caixia Fan), the Nanjing Medical Science and technique Development Foundation (QRX17006, Bin Wang), and the Nanjing Medical Science and Innovation Platform (ZDX16005, Bin Wang), Jiangsu Biobank of Clinical Resources (BM2015004), Science and Technology Bureau of Yuzhong District (20140112, Yanyan Cui)."