Acbp is essential for decidualization during early pregnancy in mice

作者全名："Zhang, Xue; Tan, Bo-Yin; Zhang, Shuang; Feng, Qian; Bai, Ying; Xiao, Shi-Quan; Chen, Xue-Mei; He, Jun-Lin; Liu, Xue-Qing; Wang, Ying-Xiong; Ding, Yu-Bin; Li, Fang-Fang"

作者地址："[Zhang, Xue; Tan, Bo-Yin; Bai, Ying; Chen, Xue-Mei; He, Jun-Lin; Liu, Xue-Qing; Wang, Ying-Xiong; Ding, Yu-Bin; Li, Fang-Fang] Chongqing Med Univ, Sch Publ Hlth & Management, Lab Reprod Biol, Chongqing, Peoples R China; [Zhang, Xue; Tan, Bo-Yin; Zhang, Shuang; Chen, Xue-Mei; He, Jun-Lin; Liu, Xue-Qing; Wang, Ying-Xiong; Ding, Yu-Bin; Li, Fang-Fang] Chongqing Med Univ, Joint Int Res Lab Reprod & Dev, Minist Educ, Chongqing, Peoples R China; [Feng, Qian] Chongqing Hosp Tradit Chinese Med, Dept Gynecol, Chongqing, Peoples R China; [Xiao, Shi-Quan] Chongqing Med Univ, Dept Reprod Med, Affiliated Hosp 3, Chongqing, Peoples R China"

通信作者："Li, FF (通讯作者)，Chongqing Med Univ, Sch Publ Hlth & Management, Lab Reprod Biol, Chongqing, Peoples R China.; Li, FF (通讯作者)，Chongqing Med Univ, Joint Int Res Lab Reprod & Dev, Minist Educ, Chongqing, Peoples R China."

来源：REPRODUCTION

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000833389000006

JCR分区：Q1

影响因子：3.8

年份：2022

卷号：163

期号：5

开始页：309

结束页：321

文献类型：Article

关键词：　

摘要："Decidualization of uterine stromal cells plays an important role in the establishment of normal pregnancy. Previous studies have demonstrated that Acyl-CoA binding protein (Acbp) is critical to cellular proliferation, differentiation, mitochondrial functions, and autophagy. The characterization and physiological function of Acbp during decidualization remain largely unknown. In the present study, we conducted the expression profile of Acbp in the endometrium of early pregnant mice. With the occurrence of decidualization, the expression of Acbp gradually increased. Similarly, Acbp expression was also strongly expressed in decidualized cells following artificial decidualization, both in vivo and in vitro. We applied the mice pseudopregnancy model to reveal that the expression of Acbp in the endometrium of early pregnant mice was not induced by embryonic signaling. Moreover, P4 significantly upregulated the expression of Acbp, whereas E2 appeared to have no regulating effect on Acbp expression in uterine stromal cells. Concurrently, we found that interfering with Acbp attenuated decidualization, and that might due to mitochondria! dysfunctions and the inhibition of fatty acid oxidation. The level of autophagy was increased after knocking down Acbp. During induced decidualization, the expression of ACBP was decreased with the treatment of rapamycin (an autophagy inducer), while increased with the addition of Chloroquine (an autophagy inhibitor). Our work suggests that Acbp plays an essential role in the proliferation and differentiation of stromal cells during decidualization through regulating mitochondria! functions, fatty acid oxidation, and autophagy."

基金机构：National Key Research and Development Program of China [2018YFC1004400]; National Natural Science Foundation of China [82104923]; Science and Technology Commission of Yuzhong District of Chongqing [20190134]

基金资助正文："This work was supported by the National Key Research and Development Program of China (Grant No. 2018YFC1004400), National Natural Science Foundation of China (Grant No. 82104923), Science and Technology Commission of Yuzhong District of Chongqing (Grant No. 20190134)."